12+-+Micro+Lab+-+Antimicrobial+Drugs_Fillable

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet     11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)     12. Petri dish     13. Culture tubes     14. Hanging drop slide     15. Durham’s tube     16. Staining rack     17. Thermostatically controlled water bath     18. Inoculating loop     19. Inoculating needle     20. Vials

General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

Questions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?

Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

Subject : Environmental Microbiology  Can u use the information given below to answer these 2 question  1.Provide an aim for this lab 2. Provide objectives  DISCUSSION QUESTIONS   What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope?  What determines the resolving power of the lens system?                                What is the limit of resolution obtainable with the light microscope?                                                 How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria?    What advantages does electron microscopy have over light microscopy?    What are disadvantages of electron microscopy over light microscopy?                               #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choic

Cause and Effect Analysis describe the effect on the test procedure and explain briefly The zones of adjacent antibiotic disks overlap. Antibiotic disk cartridge was left open for more than 7 days. Antibiotic disk was placed closely at the edge of the plate.

INSTRUCTION: Give the principle or significance of the following practices:   1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.

Instruction: answers must be in numbers. You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?

I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes   c) test tubes   d) microscope slides   2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top.   b. You notice flames coming from the tubing of your Bunsen burner

Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth? Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?

INSTRUCTION: Give the principle or significance of the following practices: NOTE: *please add references*   1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

webapps/assessment/take/launch.jsp?course_assessment_id%3D 498749 1&course_id3 111786 1&content id=D Customize Links Free Hotmail Windows Windows Media Imported From IE Importe Serial dilution QUESTION 2 What type of agar is TSA? (select all that apply) Nutrient

LAB QUESTIONS – ASSESSMENT AND CRITICAL THINKING 1. When is a simple negative stain used? 2. Why do microorganisms remain unstained in the simple negative staining procedure? 3. What information can be obtained from a simple positive staining procedure? 4. What is the difference between a simple and differential stain? 5. What is the reagent and purpose for each of the following gram stain steps? a. Primary stain b. Mordant c. Decolorizer d. Counterstain 6. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 7. What part of the bacterial cell is most involved with Gram staining, and why? 8. What cell wall component is responsible for the acid-fast property of mycobacteria? 9. Is a gram stain an adequate substitute for an acid-fast stain? Why or why not? 10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure…

Procedures/ Spccific test sample used Tests Indication/Purpose Draize Acute Skin Irritation Test Draize Eye Irritation Test Coagulation and Liquefaction of Proteins Chorioallontoic Membrane (CAM) Assay

TOPIC: KATO-THICK & KATO-KATZ TECHNIQUES What are the differences between Kato-Thick smear and Kato-Katz technique? Briefly explain each.

6 2 4 3 5 LABORATORY REPORT FORM EXERCISE 25 ASSAY OF ANTIMICROBIAL AGENTS: DISK-DIFFUSION METHODS What is the purpose of this exercise? Evaluate 1. For each of the disinfectants or antiseptics tested, determine the active ingredient. What class of inhibitory agent does it belong to and what is its mode of action? CHEMICAL AGENT Lysol Sanitizer Foaming Handwash Detergent. Desinfecting Hand Sanitizer ACTIVE INGREDIENT(S) Alhyl Dinethylaenzy/a. chloroxylenol 0.3% Alkyl, Dimethyl benzyl ammonium chlorides ethy benzyl A. C. Ethyl Alcohol 70% 1/Cleansing s Pray Hydrogen Peroxide Benzalkonium Lidocaine HC) An Septic CLASS OF AGENT MODE OF ACTION

Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstaining

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet     11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)     12. Petri dish     13. Culture tubes     14. Hanging drop slide     15. Durham’s tube

Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.

Please match the test with its description to assess your understanding of the kinds of data collected during information gathering and your understanding of the processes involved in identifying microbes from samples. 1. using macroscopic and microscopic traits for identification appearance 2. tests that determine chemical characteristics including enzyme production and nutritional requirements of the microbe biochemical tests 3. analyzing the genotype of an organism DNA profiles 4. tests the organism against known antibodies to determine if there is a reaction between the organism and the antibody immunologic testing v

困 Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following is/are TRUE the use of laboratory instruments and regarding the use laboratory instruments equipment in regards to making of culture media? " and equipment? * Air bubbles on the surface of agar plates Hot plates can be used to sterilize the inoculating loop and needle. can be removed by fanning them with the luminous flame of the bunsen burner. If the agar surface is wet, it can be dried by A drying oven is used to sterilize nutrient agar, a solid culture medium. heating at 30-40 deg C in the drying oven before inoculation is done. The refrigerator is used to grow psychrophiles. All media taken from the refrigerator should be warmed to room temperature before use, P Type here to search 7:11 pm 30/09/2021 ヘロG) ENG 近

Topic: Preparation of Microbial Suspension 5. Over-dilution with sterile water. Effect: Rationale:

Please help me answer correctly the table this is my assignment in biochem lab Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.

True or False 1. Two challenges regarding IFU are adhering with the IFU's and interpreting complex IFU. 2.  Mechanical disinfection is typically more effective than manual techniques. 3. Adding a UV disinfector to a pass-through window is NOT offer a higher level a safety for sterile processing personnel.

Topic: Pour-Plate Method  2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚CEffect: Rationale:

ISOLATE #1 Results for "Isolate #1" ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 1. Based on your results, what is the possible identification (Genus and species) of “Isolate #1"? I 9 10 11 12 3 14