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Enzymes

Decent Essays

Introduction (5 marks)

Enzymes are globular shaped proteins that are found throughout the body, with their main function being to act as biological catalysts. An enzyme can act to speed up or regulate the rate of the reaction, in order to maintain an efficient rate of biological reactions. Enzymes, whilst having an important role in the reaction of many chemicals within the body, are not consumed in the reaction, and so are able to catalyze many reactions in their life cycle. Enzymes are able to reduce the activation energy of the reaction; the energy required to break bonds between the reactants, and form new bonds in the products, which allows more product to be formed. (Marieb and Hoehn, 2010, pp.51-53).

Enzyme activity is …show more content…

Effect of temperature on alkaline phosphatase activity

| |Enzyme Activity |
|Temp |(nmoles pNP.min-1.mL-1) |
|((C) | |
|25 |15.43 |
|30 |16.83 |
|37 |19.61 |
|45 |24.11 |
|60 |14.73 |

Table 3. Effect of substrate concentration on alkaline phosphatase activity
| | |
|Substrate concentration |Enzyme Activity |
|mM pNPP |(nmoles pNP.min-1.mL-1) |
|(complete values in this column) | |
|0.25 |12.17 |
|0.5 |15.76 |
|1.0 |17.62 |
|1.5 |19.41 |
|2.0 |19.30 |
|2.5 |20.33 |

Effect of pH on activity of alkaline phosphatase
As can be seen in Figure 1. As the pH of the solution, the

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