Q: Explain how labeled antibodies are used in direct and indirect tests.
A: Antibodies or immunoglobulins are the primary line of defense in the body. They recognize and bind…
Q: Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you…
A: There are few important points : ELISA : Enzyme linked immuno sorbent assay is used in diagnosing…
Q: Why do you coat the elisa plate with albumin?
A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…
Q: All of the following are used in a direct ELISA, except:
A: ELISA is an enzyme-linked immunoassay. This assay is used for detecting the presence of antigens.
Q: What is Aseptic technique?
A: Microbiology is the study of microscopic organisms (virus, bacteria, fungi, archaea, and protozoa)…
Q: In a COVID test, why is it necessary to swab all the way at the back of the nose and throat
A: The COVID-19 pandemic has currently affected the whole world and has claimed a huge number of lives.…
Q: what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the…
A: ELISA=Enzyme linked immunosorbent assay.
Q: What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a…
A: INTRODUCTION ELISA Enzyme Linked Immunosorbent Assay, used to detect antibodies in the blood.
Q: Which tests would be faster to perform? PCR or an ELISA test?
A: ELISA ELISA is enzyme linked immunosorbent assay. It is based upon antibody-antigen interaction.…
Q: Why is important to note the Kit / Lot number and expiration date for each kit or tests done from…
A: ELISA is a biochemical analytical technique which enables us to check for the presence of a protein…
Q: Counterstaining with Hematoxylin and Eosin is an important step in which of the following…
A: Counter stains are stains that are that are in different contrast color to that of the primary…
Q: What is the label used in ELISA? Can we use different label? Why, or why not.
A: Answer
Q: During a flame test, why is it necessary to use a new splint for each element?
A: A splint is a thin wood/stick used commonly in laboratory for performing a flame test. The splint is…
Q: n not more than 100 words, What antigens/strains are used for the Weil-Felix test, and from what…
A: Well Felix test is an agglutination test for the diagnosis of Rickettsia infection. The Well Felix…
Q: List at least three different tests that fall in the direct identification category.
A: The identification of microorganisms is done on the basis of their responses/ activities with the…
Q: How do you interpret Weil-Felix Test? What are the sources of errors of the Weil-Felix Test?
A: A test used in the screening and diagnosis of typhus and other rickettsial infections is Weil-Felix…
Q: What other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA…
A: IgM/IgG immunoassay or Immunoblotting can be performed for the results obtained by ELISA test for…
Q: Why is DNA testing used for a patient with sickle cell anemia
A: Sickle cell anemia follow autosomal recessive inheritance.
Q: Briefly describe the Kirby-Bauer test and its purpose.
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Q: What are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase…
A: A nasopharyngeal swab(NPS) is a clinical test method which collects test sample from nasal…
Q: What are ELISA assays used for in labs? Give at least three examples.
A: ELISA - Enzyme-linked immunosorbent assay ELISA is an analytical immunological technique to detect…
Q: Briefl y describe the Kirby-Bauer test and its purpose.
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Q: Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification…
A: When a person gets infected with the virus such as Human Immunodeficiency Virus, the body develops…
Q: What are the advantages and disadvantages of using the E-test?
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Q: If PBR323 is used instead of pUC18, is blue white screening applicable? Justify your answer.
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Q: What color tube is used for creatinine test?
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Q: what are the important principle in polyphenoloxidase test
A: Polyphenols, as the name sounds is a large family of organic compounds that are characterized by…
Q: A positive ELISA assay usually results in __________. decreased motility fluorescence…
A: ELISA is an enzyme linked immunosorbent Assay which is a biochemical test used to detect the…
Q: What is an antigen? What is an antibody? Why does each test include a control line?
A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…
Q: What would you use for a positive control in any ELISA test? What would you use for a negative…
A: While doing Immunology experiments, scientists often use ELISA tests. The full form of ELISA is an…
Q: Among these protein assays, which is appropriate for solutions with high protein concentration or…
A: Protein assay is a quantitative analytical technique. It is carried out to determine the amount of…
Q: discuss the purpose of the Methyl Red and Voges-Proskauer Test. What makes each one unique and why
A: Methyl Red (MR) and Voges-Proskauer (VP) broth are used for the tests for Methyl Red and…
Q: Why are two negative controls included in an ELISA test
A: ELISA TEST (Enzyme linked immunosorbent assay)is used in aboratory test to detect antibodies in…
Q: If you forgot to wash the wells after you added the secondary antibody, what would you expect to see…
A: ELISA stands for enzyme-linked immunosorbent assay. It is a plate-based assay technique designed for…
Q: 6. assay kits that determine the amount of glucose present
A: To give proper treatment for any disease condition it is very much important for the nurses and…
Q: Why do you think it is called indirect ELISA?
A: ELISA is used to detect the antigens or proteins by using specific antibodies against them.…
Q: If you were using the ELISA to look for the presence of antibodies and the sample gave a negative…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: What tests are included in a CBC? Give the normal value,and name a disorder that would cause an…
A: Complete blood test It is the test to perform to evaluate various parameters of blood cells. Purpose…
Q: DQA1 test chosen for DNA analysis
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Q: The direct ELISA test requires only one antibody only one antigen both primary and…
A: The direct ELISA test required only one antigen. In a direct ELISA an antigen is immobilized…
Q: What is the effect of not adding the primary antibody to the ELISA reaction?
A: ELISA or Enzyme-linked immuno sorbent assey or immunoassay (EIA) was first described by Engvall and…
Q: Why does RNA give a positive result to Biuret's test?
A: Biuret test is a chemical test used for detecting the presence of peptide bonds. In presence of…
Q: In five sentences, summarize how an ELISA assay “works.” How is the test quantified?
A: Elisa is enzyme linked immunosorbant assay .it is a biochemical immuno assays
Q: What does BCR ABL positive mean?
A: The cancer of the white blood cells is known as Chronic myelogenous leukemia (CML) in medical terms.…
Q: What about a Katz Test? How is this different or similar to Barthel’s Test?
A: Katz activities of daily living (ADL) test is a gold-standard test used to quantify the functional…
Q: Outline the principle behind the following techniques:…
A: Immunofixation is an immunological technique that involves antigens and antibodies. Southern blot is…
Q: What is the basis for the technique called ELISA?
A: Engvall and Perlmann initially described the enzyme-linked immunosorbent test in 1971, and it is…
Q: Would any of the above give a positive Lieberman-Burchard test? Explain your answer.
A: The endocrine system is a vital biological system that produces various hormones. These hormones…
Q: What are the two types of ELISA methods and how do they work? What is a chromogen?
A: Enzyme-linked immunosorbent assay (ELISA) is a non-isotopic immunoassay. An enzyme is utilized as a…
1) What are you testing in the picture above?
2) How would you interpret this test?
3) What is the basis for this type of test?
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- What is the Kirby-Bauer Test? Describe it and explain how you would interpret the results.A fellow classmate comes to you to ask your opinion about the following result from this SIM test: (Uploaded Picture). a) Provide a full interpretation and analysis of the results this test. b) Your classmate is not fully convinced about the black coloring seen in this result. Please suggest another type of method that would help confirm this result and what explain what the result should show.What is the principle behind Liebermann-Burchard test? And What is the clinical significance of Liebermann-Burchard test?
- What is the purpose of conducting a modified Furter-Meyer Test? What is the principle behind this test? How could you identify the positive result for this test?3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?If you are using sensitivity and specificity to evaluate how well a dichotomous test (like a field sobriety test or home pregnancy test) is working, ideally what do you hope to see? Choose the correct answer and explaing why: a) Low specificity and low sensitivity. b) High specificity and high sensitivity. c) Low specificity and high sensitivity. d)_High specificity and low sensitivity.
- What is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test? How did you figure out that this test had a positive result?Can you show the step by step process of Voges Proskauer Test using a schematic diagram?How do you interpret Weil-Felix Test? What are the sources of errors of the Weil-Felix Test?
- What is the benefit of doing a modified Furter-Meyer Test? What is the premise of this experiment? How would you know if this test yielded a positive result?What are the different methods of sensitivity testing? Discuss brieflyWhich is better? A test with high sensitivity or a test with high specificity?