1. A wild-type strain of bacteria produces a protein with the amino acid proline (Pro) at one site. Treatment with nitrous acid causes a C to change to a U. Two different mutants are produced after nitrous acid treatment of the bacteria. One has a serine (Ser) in place of the proline, an the other has a leucine (Leu). Further treatment of the two mutants with nitrous acid produces new mutant strains, both with phenylalanine (Phe) at the site. Treatment of the phenylalanine carrying mutants produces no change. ·ច Pro. a. Ser Leu -Phe—>Phe C fuuu UUC ULA UUG CUU CUC CUA CUG AUU AUC AUA AUG GUU GUC G GUA GUG Phe Lau Leu 1 Mot 3 C UCU- UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG OCU GCC GCA GCG Ser Pro Thr Als A UAU UAC UAA UAG CAU CAC CAA CAG AAU AAC GAU GAC Tyr GAA GAG Stop His Gin AMA AAG Lys Aan Asp Glu UQU cy UGC UGA-Stop UGG G COU COC CGA COG AGU AGC AGA AGG GGU GOC GGA GGG b. Explain why further treatment of the phenylalanine mutants with nitrous acid produces no change. Arg Ser Arg Use appropriate codons to show how these mutations occurred. Assume only a single nucleotide change occurs with each treatment of nitrous acid.
1. A wild-type strain of bacteria produces a protein with the amino acid proline (Pro) at one site. Treatment with nitrous acid causes a C to change to a U. Two different mutants are produced after nitrous acid treatment of the bacteria. One has a serine (Ser) in place of the proline, an the other has a leucine (Leu). Further treatment of the two mutants with nitrous acid produces new mutant strains, both with phenylalanine (Phe) at the site. Treatment of the phenylalanine carrying mutants produces no change. ·ច Pro. a. Ser Leu -Phe—>Phe C fuuu UUC ULA UUG CUU CUC CUA CUG AUU AUC AUA AUG GUU GUC G GUA GUG Phe Lau Leu 1 Mot 3 C UCU- UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG OCU GCC GCA GCG Ser Pro Thr Als A UAU UAC UAA UAG CAU CAC CAA CAG AAU AAC GAU GAC Tyr GAA GAG Stop His Gin AMA AAG Lys Aan Asp Glu UQU cy UGC UGA-Stop UGG G COU COC CGA COG AGU AGC AGA AGG GGU GOC GGA GGG b. Explain why further treatment of the phenylalanine mutants with nitrous acid produces no change. Arg Ser Arg Use appropriate codons to show how these mutations occurred. Assume only a single nucleotide change occurs with each treatment of nitrous acid.
Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter8: Dna Structure And Function
Section: Chapter Questions
Problem 4DAA: HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952...
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