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- 1. List the different reagent strip tests with their principle 2. Enumerate 5 clinical significance for each reagent strip tests2. Make a viable count of the donor culture by spot inoculation of the nutrient agar plate WITHOUT antibiotics as follows: • Prepare a 10 fold serial dilution (to 10-7) of the DONOR culture in sterile saline. • Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of 10-1 10-2 10-3 104 10-5 10-6 10-7 which other | dilution? • Clearly label the base of the nutrient broth agar plate as shown in the diagram below. Include your initials, the date, and what the plate contains (donor viable cell count). • Carefully dispense 3 x 10 µl drops of the 104, 10-5, 106 and 10-7 dilutions of the culture within quarter areas of the agar plate then replace the lid. Allow the inocula to soak into the agar before inverting the plate for incubation. • What is the purpose of this step? 492. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.
- 1. Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.1. Conclude regarding the chemical composition of the formation of film in fresh milk in biuret test and acrolein test.list the reagents used in the IMViC tests and what they detect
- In your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?
- 1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes2. What microorganisms other than coliforms are likely to give positive presumptive test?1. Calcium Chloride. Dosage forms, qualitative and quantitative analysis.