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- The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of five 50.0-mL volumetric flasks. Various volumes of a standard containing 12.2 ppm Cr were added to the flasks, following which the solutions were diluted to volume. Unknown,mLStandard, mLAbsorbancc 10.00.00.201 10.010.0 0.292 10.020.0 0.378 10.030.0 0.467 10.040.0 0.554 (a) Plot the data using a spreadsheet. (b) Determine an equation for the relationship between absorbance and volume of standard. (c) Calculate the statistics for the least-squares relationship in (b). (d) I)ctcrmine the conccnt ration oÍCr in ppm in the sample. (e) Find the standard deviation of the result in (d).What is difference between SPE and DSPE?Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.
- Seawater was spiked with phosphate to achieve a quantifiable range of phosphate concentrations by the spectrophotometer. What components of the spectrophotometer or sample preparation could be changed that would improve detection limits?What are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in HPLC?Discuss the advantages and disadvantages of sequential versus simultaneous multichannel ICP spectrometers.
- Frankie determined the trendline for their Calibration Curve for the relationship between Absorbance and [FeSCN2+] was y = 3823.3 x − 0.0127 During Part B, Frankie measured the absorbance of Equilibrium Solution 3 as 0.292 a.u. (absorbance units). Question 1: What is the equilibrium concentration of the [FeSCN2+]? Anjelica used the absorbance of 0.449 a.u. to determine the equilibrium concentration of [FeSCN2+] for Run 5 was 1.17 x 10-4 M. Previously, Anjelica calculated the initial concentrations of [Fe3+] = 1.00 x 10-3 M and [SCN-] = 1.00 x 10-3 M. Question 2: What are the equilibrium concentrations of [Fe3+] and [SCN-]? Please answer completely all questions will give rating surelyTrace amounts of rare clements are found within groundwater and are of interest to goochemists. Earopium and terbium are 30 - lanthanide-scries clements that can be measured from the intensity of their fluorescence emittcd when a solution is illuminated with ultraviolet radiation. Certain organic 25 - compounds that bind Eu(III) and Tb(IIl) enhance the cmission, and substances found in natural waters can decrease 20- the cmission. For that reason it is necessary to use standard Eu (II) additions to the sample to correct for such interference. The graph shows the result of such an cxperiment in which the 15- concentration of Eu(III) and Tb(III) was mcasured in a sample of groundwatler. The x-intercept of the Eu(III) plot is 10- -23.1 ml. and the x-intercept of the Th(III) plot is -9.91 ml. In cach case, 10.00 ml. of the sample solution and 15.00 ml. 5- Tb (III) of an organic additive were added to a scries of live 50 ml. volumctric flasks. The standard Eu(lI) or Th(III) solution (0,…A 10 cm 3 air saturated Fricke dosimetry solution in a 1 cm diameter tube is irradiated for 10 min in a 6~ source of gamma rays. The optical density measured at 304 txm in a 1 cm light path at 30~ was 0.260 after the completion of irradiation and 0.003 before irradiation. (a) What is the total dose in gray absorbed by the solution?(b) If 10 cm 3 of methanol is irradiated in the same tube for the same 10min, what is the absorbed dose in gray. The Z/A for methanol is 0.562 and that for the Fricke dosimeter is 0.553. Explain any assumption you make to solve the problem.
- You have measured the blank for a determination of arsenic in food samples by hydride-generation atomic fluorescence spectrometry. The blank values are: 0.23 ppb, 0.14 ppb, 0.16 ppb, 0.28 ppb, 0.18 ppb, 0.09 ppb, 0.10 ppb, 0.20 ppb, 0.15 ppb, 0.21 ppb As. What is the LOD?List three typical examples applications of SFC analysis.(Standard addition) Tooth enamel consists mainly of the mineral calcium hydroxyapatite, Ca10(PO4)6(OH)2. Trace elements in teeth of archeological specimen provide anthropologists with clues about diet and diseases of ancient people. Student at Dankook University measured the trace element strontium (Sr) in enamel from extracted wisdom teeth by atomic absorption spectroscopy. Solutions were prepared with a constant total volume of 10 mL containing 0.750 mg dissolved tooth enamel plus variable concentrations of added standard Sr. Added Standard Sr (ng/mL=ppb) 0 2.50 5.00 7.50 10.00 Emission Signal 28.0 34.3 42.8 51.5 58.6 (a) Make a graph showing the experimental data and the calculated straight line. (b) Find the concentration of Sr in the tooth ennamel in parts per million = μg/mL.