2. One of the common methods used to determine phosphorus in urine isto treat urine with molybdenum(VI) resulting in a complex compoundof 12- molybdophosphate and then reduced using ascorbic acid produces a blue-colored compound called molybdenum blue (max = 650 nm). A patient's urine sample collected over 24 hours produced 1122 mL of urine. A total of 1 mL of the sample was treated with Mo(VI) and ascorbic acid and diluted until it reached a volume of 50 mL. A calibration curve is also prepared by treating 1 mL of standard phosphate solution in the same way. The absorbance of the standard solution and the sample was then measured at a wavelength of 650 nm and produced the following data: Solution Absorbance at λ 650 nm Phosphate 1 ppm Phosphate 2 ppm Phosphate 3 ppm Phosphate 4 ppm Urine sample 0,230 0,436 0,638 0,848 0,518 a. Create a calibration curve for the data and determine the slope, intercept, and R values of 2! Determine the concentration of phosphates found in urine samples in units of ppm and mM! b. Determine the mass of phosphate excreted by the patient for 24 hours?

2. One of the common methods used to determine phosphorus in urine isto treat urine with molybdenum(VI) resulting in a complex compoundof 12- molybdophosphate and then reduced using ascorbic acid produces a blue-colored compound called molybdenum blue (max = 650 nm). A patient's urine sample collected over 24 hours produced 1122 mL of urine. A total of 1 mL of the sample was treated with Mo(VI) and ascorbic acid and diluted until it reached a volume of 50 mL. A calibration curve is also prepared by treating 1 mL of standard phosphate solution in the same way. The absorbance of the standard solution and the sample was then measured at a wavelength of 650 nm and produced the following data: Solution Absorbance at λ 650 nm Phosphate 1 ppm Phosphate 2 ppm Phosphate 3 ppm Phosphate 4 ppm Urine sample 0,230 0,436 0,638 0,848 0,518 a. Create a calibration curve for the data and determine the slope, intercept, and R values of 2! Determine the concentration of phosphates found in urine samples in units of ppm and mM! b. Determine the mass of phosphate excreted by the patient for 24 hours?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.19QAP

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