2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K₂ Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaCH₂COO SAD Boric acid, H3BO3 5.81 x 107 NaH2BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K₂ Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaCH₂COO SAD Boric acid, H3BO3 5.81 x 107 NaH2BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter14: Chemical Equilibrium
Section: Chapter Questions
Problem 14.97QE: According to the Resource Conservation and Recovery Act (RCRA), waste material is classified as...
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