Answered: 2. There are two major forces…

2. There are two major forces propelling the separation in paper chromatography. Which was more important in this procedure? How did you come to this conclusion?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Related questions

Q: Give typed full explanation If hypertension is left untreated, the risk of dying from cardiac…

A: It is a condition of increased blood pressure. In general, blood pressure reading of 130/80 mm Hg…

Q: 5. Enumerate kidney function tests. Discuss tests to assess Glomerular filtration rate.

A: The kidneys are vital organs that play an essential role in filtering waste products from the blood,…

Q: Use the Saccharide to draw the remaining forms (Haworth and Linear) of the sugar

A: Here we are given the 4C1 chair conformational form of a saccharide (sugar). The inner chair…

Q: Block diagrams representing the general structures of two types of lipids are drawn. Which terms…

A: The lipid given to us is composed of 2 types of molecules; the one GREEN bar represents a type of…

Q: Inulin is a type of fat. is a synbiotic. is usually taken to relieve gas. is a fructose polymer. is…

A: Carbohydrates are one of the three main macronutrients that provide energy to the body. They are…

Q: A violation of the First Law? The complete combustion of glucose to CO 2 and H 2 O yields 30 ATP, as…

A: The First Law of Thermodynamics, which states that energy cannot be created or destroyed, only…

Q: Which peptide contains amino acids with side chains such that the peptide would exhibit…

A: When 2 uncharged atoms comes close to each other, their electron clouds influence each other. This…

Q: Explain the process of transcription. Add a note on its inhibitors.

A: Transcription is the process by which genetic information stored in DNA is converted into RNA. This…

Q: A) Please explain the differences between these isozymes as shown by the Michaelis- Menten plot. B)…

A: Isoenzymes : The enzymes that are characterised by the same function but possess different…

Q: Select the statements that correctly describe SDS‑PAGE. SDS‑PAGE is a protein used as a loading…

A: SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a common technique used in…

Q: (a) ribose 5-phosphate from glucose 6- phosphate without the concomitant generation of NADPH? (b)…

A: Stoichiometry involves the relationships between the reactants and/or products in the chemical…

Q: Bound bilirubin (conjugated or direct bilirubin): structure, clinical and diagnostic value.

A: Bound bilirubin is a type of bilirubin that has been chemically altered or conjugated with…

Q: 11. Which of the following phospholipids is localized to a greater extent in the outer leaflet of…

A: The distribution of phospholipids in the membrane lipid bilayer is asymmetric, meaning that certain…

Q: Some E. coli mutants contain defective DNA ligase. When researchers expose these mutants to…

A: Semi-conservative replication is a mode of DNA replication in which each newly synthesized DNA…

Q: In my text book, three types of inhibitions: 1, competitive inhibition 2. non-competitive…

A: Enzymes are high molecular-weight proteins that catalyse biochemical reactions. They contain an…

Q: Lysozyme is a Lipid Carbohydrate Protein Nucleotide

A: Lysozyme is an enzyme that catalyses the hydrolysis of β-1,4-glycosidic linkages. Hydrolysis means…

Q: the 10 mg/mL sample of lysozyme shows a lower activity than 2mg/mL lysozyme sample (refer to the…

A: The most likely explanation for the lower activity of the 10 mg/mL lysozyme sample compared to the 2…

Q: Draw the structures of the products formed by hydrolysis of the following tripeptide at…

A: Hydrolysis of the tripeptide Val-Gly-Ile will be the three respective amino acid residues of the…

Q: a) What type of polymer is being formed in Reaction #3? b) Name an enzyme that can catalyze…

A: In human body, many polymers are being synthesized from monomers. One such polymer is nucleotide…

Q: Can you draw the steps, metabolic intermediates, and by-products of glycolysis and the TCA cycle

A: Cellular respiration is a collection of three metabolic pathways that generate ATP the energy…

Q: Diametrically opposed . Phosphorylation has opposite effects on glycogen synthesis and breakdown.…

A: Glycogen breakdown and synthesis are reciprocally regulated by a hormone-triggered cAMP cascade…

Q: Draw the chair conformation of the whole of O-linked oligosaccharide pictured below, including the…

A: NeuNAc stands for N-Acetylneuraminic acid. GalNAc stands for N-Acetylgalactosamine. Gal stands for…

Q: 21. Dietary Sources, RDA factors affecting absorption, functions and deficiency manifestations of…

A: In this answer, we will discuss the important mineral calcium, which plays a crucial role in…

Q: 5. The proteins listed below are separated by (1) isoelectric focusing (IEF) followed by (2) sodium…

A: Since you have posted a question with multiple sub parts, we will provide the solution only to the…

Q: Identify (name) each of the following two monosaccharides A and B. НО ОН A ОН ОН -ОН НО НО Но ОН B…

A: In the general chair conformation of pyranoses, the 'O' atom within the ring would be on the top…

Q: At different glucose concentrations (2 mol of glucose, 4 mol of glucose, 8 mol of glucose), at what…

A: We know that glucose is the most favored respiratory substrate of our body. Glucose is efficiently…

Q: The buffer systems of blood, their role in maintenance of acid-base homeostasis

A: A buffer is a solution that can resist changes in pH when small quantities of acid or base are added…

Q: Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any…

A: DNA can be extracted from any tissue or cell. There are four basic steps of DNA extraction. They…

Q: Can you explain why when the pH level was changed from 0 to 5, the ATP production was affected…

A: ATP synthesis can occur by 2 modes: Substrate level phosphorylation Oxidative phosphorylation…

Q: 2. a) Outline the general mechanism of a coenzyme B12-dependent enzyme which catalyzes the following…

A: We are looking at a biochemical reaction where a 1,2-diol is converted to an aldehyde along with the…

Q: Explain about Cytochrome P450 in detoxification.

A: Cytochrome P450 (CYP) is a group of enzymes that plays a crucial role in the metabolism of…

Q: 1. Using phycocyanin as a model, what types of agents can you say will permane protein? MINAYOOOYH…

A: Phycocyanin is a protein which capture light energy. Hence, it is a accessory protein in…

Q: 14. The monosaccharide units are linked by 1→ 4 glycosidic linkage in (A) Maltese (B) Sucrose (C)…

A: Carbohydrates are sugar molecules. Saccharides are another name for sugars. Monomer units are single…

Q: Given the reaction below, what major class of enzyme will catalyze such reaction? ОН HO‘‘ ОН „ОН "ОН…

A: Enzymes are classified into the following six groups: 1. Oxidoreductases — catalyze oxidations…

Q: 3. Free bilirubin (unconjugated or indirect bilirubin): structure, clinical and diagnostic value.

A: Free bilirubin, sometimes referred to as unconjugated or indirect bilirubin, is a byproduct of the…

Q: raw TCA Cycle. Please make sure to state all the enzymes and co-factors for each step of the…

A: In glycolysis, a 6-carbon molecule of glucose-6-phosphate is broken down into 3-carbon pyruvate. It…

Q: Consider the amino acid valine. Note: Reference the Part: 0/4 Part 1 of 4 Naturally-occurring amino…

A: Valine is one of the 20 amino acids that are used to build proteins in the body, and it has a…

Q: 4. Describe the detoxication of ammonia by urea cycle. Explain its regulation and disorders.

A: Ammonia is a toxic waste product that is generated during the metabolism of nitrogen-containing…

Q: A drug which prevents uric acid synthesis by inhibiting the enzyme xanthine oxidase is (A) Aspirin…

A: Catabolism of guanine and adenine generates xanthine. Xanthine oxidase then catalyzes the conversion…

Q: compute the yield of ATP when one molecule of hexanoic acid (6:0 fatty acid) is completely oxidized…

A: Aerobic respiration is a metabolic pathway that breaks down organic molecules, such as glucose and…

Q: Propose a full mechanism for the formation of the diene. Show curved arrows to illustrate electron…

A: Here we are to decipher the reaction mechanism for the conversion of lysine to…

Q: Which of the following is/are descriptive of all globular proteins?

A: Globular proteins are one of the two main types of proteins found in living organisms, the other…

Q: Propose a plausible enzyme mechanism for triose kinase, the enzyme that catalyzes the conversion of…

A: Triose kinase, like other kinases, catalyses the transfer of phosphate group from ATP to acceptors…

Q: An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by…

A: In the enzyme assay conducted here, we are trying to figure out how the kinetics of the reaction is…

Q: 11.30) Identify the following as properties of amylose, amylopectin, both amylose and amylopectin,…

A: Polysaccharides are complex biomolecules composed of carbohydrates made up of long chains of…

Q: What is the Keq of Reaction #3? Show your work & clearly label your answer. Is Reaction #3…

A: In a general reaction such as: aA + bB ⇌ cC + dD At equilibrium (steady state), the concentration of…

Q: During SDS-PAGE, the charge on glycine in the separating gel is ____________ while the charge on…

A: SDS-PAGE, or sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used to…

Q: Explain Metabolic changes during starvation.

A: Starvation is a condition that occurs when an individual experiences a prolonged period of…

Q: Two amino acids are shown below. Name them in the blanks, and then draw the structure of the…

A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…

Q: Give the omega-n designation for each acid. Part 1 of 2 gadoleic acid CH₂(CH₂), CH=CH (CH₂),COOH…

A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons. The…

Question

Based on separating amino acids by paper chromatography

Proteins and Amino Acids
2. There are two major forces propelling the separation in paper chromatography. Which
was more important in this procedure? How did you come to this conclusion?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction

VIEW

Step 2: Explanation

VIEW

Solution

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

Nonspecific elution of affinity bonded macromolecules is used in affinity chromatography explain why?
Can Cells Be Separated into Their Component Fractions? How? Centrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. What is Chromatography? Can proteins be purified and separated through Chromatography? Explain your answer. Define the following: SDS Polyacrylamide-Gel Electrophoresis and Immunoprecipitation
Thinking about the complexity of biochemical systems as they relate to the human body and the specificity of DNA, why can we not describe the “average” behavior of a DNA molecule?
TRUE OR FALSE 1. Nitrogenous bases cannot undergo electrophilic aromatic substitution.2. Cytosine forms three hydrogen bonds with guanine while Adenine forms two hydrogen bonds with Thymine in themessenger RNA.3. The glycosidic bond in nucleotides is stable in dilute ammonium hydroxide solution and dilute Sodium bicarbonatesolution.4. In gel electrophoresis, a DNA is expected to migrate towards the cathode.5. Tautomerism is a phenomenon observed in both purine and pyrimidine bases.
Question: Which base (A, C, T or G) corresponds to X in the unknown? I did the experiment, got the data below, and calculated the binding constants. But I am TOTALLY lost as to how to figure this out! I don't even know what steps I would take. Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M 3.322 C & X [C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M 1.409 T & X [T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M 2.333 G & X [G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M 2.633
(b) Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations weredetermined by the Bradford protein assay. Assay conditions employed in the two labs (pH, temperature,etc.) were also identical. What would be the most plausible cause for the discrepancy in the Vmax valuesfor the compound I? Explain.Recall that the Bradford assay measures total protein amounts in sample solution based on complexformation between a dye and proteins. Also, the assay solution used in both labs does not contain anyinhibitors.
briefly explain the physical mechanism by which amino acids are separated during paper chromatography.
Movement of Dyes in an Agarose Gel.This problem tests your ability to understand basic concepts of agarose gel electrophoresis.The gel is loaded from lane 1 at the top to lane 7 at the bottom with: bromophenol blue, safranin O, orange G, crystal violet, xylene cyanol, methylene blue, and the unknown.Lines have been added to the gel to help you locate the center of the wells. Which known dye samples are positively charged? Which known dyes are in the unknown sample in lane 7?
Confirm Regularly bring your favourites, password... of 45 OCR 2021. You may photocopy this page. 7 of 46 Create 4 Which of the following nucleotides contains uracil? A B NH H,C. ofo HN HO-P-O- HO-P-O- OH ОН ОН OH 'NH HO-P- NH, HO-P-O OH ОН ОН OH Your answer An anticodon seguence of five successsive tRNA molecules involved in protein sunthosie was ar
The purification continues with a cation exchange step in which the positively charged cytochrome C protein is separated from negatively charged DNA and other proteins. The cation exchange eluate (volume of solution collected) had a total volume of 42.0 mL and a 1.0 mL aliquot was set aside for further analysis. The following data was obtained from the 1.0 mL aliquot to quantify the protein amount and purity: The absorbance at 410 nm of the aliquot was diluted 5-fold was 0.474 (1 cm pathlength). The absorbance at 595 nm from a 1.0 mL Bradford Assay solution that was diluted by 100-fold from the aliquot was 0.195 (1 cm pathlength). Using the information given, Calculate the total protein amount in mg from the absorbance at 595 nm. Calculate the cytochrome C amount in mg from the absorbance at 410 nm using Beer’s Law.
Could you please help me with my reaction paper. Make a simple reaction to this. Thank you
FLUORESCENCE BINDING a) In the DAPI DNA lab, concentration of DAPI and DNA was determined to carry out analysis. Here is the scenario: 3.50 mL of DAPI at 25 micromolar was placed in the cuvette. It was titrated with a 150 microgram/mL DNA stock. It took 230 microliters of DNA stock to reach saturation. What is the DAPI molarity at saturation in the cuvette? What is the DNA molarity (in terms of base, 1 base = 325 g/mol) at saturation in (i) (ii) cuvette? im DNA = 150 × 10 g/mL 3.5-CAP 25 μM the 230 μL