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- Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
- 10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure culture be obtained from a mixture of bacteria? 12. What is a selective agar? Why can it be of importance?Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. Gram positive Gram negative 2. Mordant was skipped Gram positive Gram negative 3. Decolorizing step was omitted Gram positive Gram negative 4. counterstain was not used? Gram positive Gram negative 5. Crystal violet and safranin was swapped in position. Gram positive Gram negative
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiINSTRUCTIONS: Read each sentence carefully Encircle the biological techniques that are being described. 1. Itis also known as sterile technique. Aseptic technique Disinfection Answer: 2. It is described as the complete removal of microorganisms found in a specimen or sample. sterilization Disinfection Answer: 3. It is described as the elimination of pathogens that reduce the microbial contamination. sterilization Disinfection Answer: 4. This is described as the elimination of life processes in a specimen. Killing Fixation Answer: 5. Clearing is also known as Dehydration Dealcoholization Answer:Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood Agar - 48 hours Growth on Chocolate Agar Results: Growth on Blood Agar 48 hours Growth on Chocolate Agar
- indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассeptor 1 Oxidase test Tryptic 24 – 48 hours soy agar (TSA), QxiDrop 2 Catalase Охудen test N/A Nitrate 37 reductase N/A testWhat is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?