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- Sugar fermentation (Mannitol): 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in sugar fermentation 4. Explain the interpretations in sugar fermentationO The following is a Mannitol Salt Agar plate. What does the growth on this plate indicate? www TAN ASM's MicrobeLibrary Reynolds lactose metabolism tolerance of the dyes and chemicals in the agar tolerance of the salt mannitol metabolismGive the components, properties and special considerations in preparation (if there's any) on the following media 1. MacConkey Agar 2. EMB Agar 3. HEA 4. SSA
- Why is Phenol red being utilized in MacConkey agar? Choose among the choices and further explain it.A. Inhibit microscopic organismsB. Detect the production of gas by-productsC. Conduct the sulfate reduction testD. Detect the acid reaction of lactose fermentation2. What is the cfu/g or cfu/mL in each sample if the following counts were obtained after spread plating on Potato Dextrose Agar with chloramphenicol? Mongo bread 221, 257, 292 Melon juice TNTC, TNTC, TNTC Dilution 10-1 59, 41, 30 125, 132, 124 10-2 No growth 19, 17, 15 10-3Which can infect hur Table 1: Absorbance and appearance of broth tubes at various temperatures. E. colin B. stearothermophilus Temperature°C Absorbance Visual Absorbance Visual 4 0.04 Clear 0.06 Clear tre Light turbid Very turbid Almost clear 25 0.66 0.09 Clear Light turbid Very turbid 37 1.48 0.55 which 55 0.25 1.29 oledzethe mes he alhe ive to edeut et brahell bras atnstooinieib o ho leviviue zwolls ti saun
- MacConkey agar Results: Complete the below chart based on the image. **** to TAREM.. MANGE B and pr ARAS FURR Colony Color Lactose Fermentation + or -1. In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like Ca, Fe, from? 2. During sterilization, pH of culture media may change. How can this be fixed? 3. How does pH affect the shelf life of unsterilized media?1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses. 2.
- Given the following raw main ingredients choose rhe possible food preservation method to be used and the product that can be made1. MEDIA. Match the name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer.a. Kligler’s iron agar 2,3-butanediol fermentationb. MR-VP broth carbohydrate fermentationc. phenol red lactose casein hydrolysisd. SIM medium citrate utilizatione. Simmon’s citratr hydrogen sulfide productionf. skim milk agar mixed-acid fermentationg. spirit blue agar triglyceride hydrolysish. tryptone broth tryptophan degradation12. Differentiate exudate from transudate in terms of: Exudate Transudate Cause Appearance Specific Gravity Spontaneous Coagulability Protein Content Cells Bacteria