6. Calculate the dilution factors for each of the tubes A through D below. Notice that this is an example of variable dilution factor between dilutions. Pay attention to the volumes transferred and the volumes in each tube. SHOW YOUR WORK and give your answer as a ratio (1:X). Stock Let's review some arithmetic and dilution problems 10 μ. 990μl + 10 A 1 με 999μl +1 B 10 μ. 90μl +10 C 1 μ. 9μl +1 D
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- Calculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uLA nurse wants to know the what volume of Water for Injection, BP to be added to a vial containing 500mg doxorubicin to produce an injection with a concentration of 2.5 mg/mL. The displacement volume of doxorubicin is 0.3 mL/10 mg.1 2 3 1 point Mike needs to pipette 25 uL of solution. Which pipette should he use to pipette the most accurate amount of solution? P1000 OP20 1 point You need to pipette a solution of 150 uL. What pipette will you use? What will you set this pipette to (i.e. what will the numbers read on the pipette?) P1000; 0150 2000 P10 P200 ▶⠀ ⠀ > > n P10; 015 1 point Which of the following are best practices for using a micropipette? Select all that apply. Never exceed the upper or lower limit of the pipette If only pipetting a little extra, use a pipette outside of the range limit Point the pipette tip upwards Use a new tip for different liquids When withdrawing liquids, always release the plunger slowly P200; 150 P20; 150
- Calculate the final dilution in the following dilution series: a. 1 mL in 9 mL dilution & 100uL spread onto the plate b. 1 mL in 9 mL dilution followed by a 100ul in 900ul dilution & 100uL spread onto the plate (note bacteria are counted on plates as cfu/mL)Propose a serial dilution that would give total dilution of 1/158000000 (six zeros) Each tube in your dilution series must have a volume of 50 ml or less.1200 ml of iv fluid must be administered to the patient over an 8 hour period. If the calibration rate is set a 8 drops/ ml, how many drops per minute will be administered to the patient?
- You need to administer furosemide (Lasix) 2 mg/minute via continuous IV infusion on a controller. Pharmacy has sent a bag of Lasix 400 mg diluted in D5W 250 mL. How many milliliters per hour will you set on the controller? Fill in the blanks. Answer: ml/hrexplain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088900mL mL of a dextrose solution is to be infused at 50 mL/h starting at 1600 hours. At what time will this IV be completed? Round your answer to the nearest minute
- 750mL mL of a dextrose solution is to be infused at 45 mL/h starting at 1600 hours. At what time will this IV be completed?(e) Complete the following table: Number printed on the micropipette's end (its plunger) 20 20 200 1000 1000 Number showing in the micropipette's display window OTOHOSHOSHOOO 0 7 0 1 0 5 1 0 5 1 0 0 0 2 1 Volume that the micropipette is set to draw up (include units in your answer) 6) Before using a micropipette, you have to first place a 7) Shown below are the steps required to transfer fluid from one tube to another using a micropipette. Write numbers in the blanks to indicate the correct order in which the steps should be performed. Slowly release the micropipette's plunger. Place the micropipette tip into the tube containing fluid you wish to draw up. Push down on the micropipette plunger until it reaches the second stop. Place the micropipette tip into the tube that you wish to expel fluid into. Push down on the micropipette plunger until it reaches the first stop. 8) Suppose that you're getting ready to draw fluid into a micropipette. To do so, you push the micropipette's plunger…пgусетае mez i9owss2 to yliznsb 9di 11.Which of the following devices will allow measurement of a liquid's volume with the greatest accuracy and precision? a. 50 ml cylinder graduated in 0.5 mL increments b. 100 mL cylinder graduated in 1 mL increments c. 100 mL cylinder graduated in 10 mL increments d. 20 mL cylinder graduated in 5 mL increments I. chose A whch is corre t Water Mo Cohesion r