6. Optimization of an analytical method is performed during validation. True or False?
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STEM Workplace Practices Q6
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- Describe the role of the “surprise” protocol in the experimentaldesign.Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Explain the concept of method validation by describing five importantvalidation facets?
- Discuss the different designs for collecting the information desired in a Randomised Controlled Trial (RCT).The following are words used in animal testing and its alternative methods. Define the following terms - a. Vivisection b. Optogenics c. In vivo d. In vitro cell culture e. In silico computer simulation f. Pharmacokinetics g. Pharmacodynamics h. Tissue culture i. Phototoxicity j. 3D cell cultures10) List down 3 different test animals and give samples for which purposes they can be used in testing of a designed biomaterials? 11) What does FDA abbreviation stand for? 12) Order for the below given steps prior to FDA approval ? Controlled clinical trials In vitro testing (“in glass") In vivo testing w/animal models of disease In vivo testing w/healthy animals
- Describe some sources of potential bias in biomedicalresearch. What are some ways of minimizing such bias?state what the ethical problems are in the case of covid 19 of the challenge testing? List Three reason? what are the problem? How are those terms problems ? What are the problems with them?Define the following terms:• Preclinical Trial• Clinical Trials• FDA Review• Lead discovery• Molecular target-based screening• Phenotypic screening
- What is metrology? How does metrology relate to Analytical Biotechnology? Relate the terms error and uncertainty to the concepts of precision and accuracy. What are the major parameters of instrumental response?• In your own words...how the heck do primers work? What are they actually doing that enable us to use them as a tool in diagnostic science?If you are using sensitivity and specificity to evaluate how well a dichotomous test (like a field sobriety test or home pregnancy test) is working, ideally what do you hope to see? Choose the correct answer and explaing why: a) Low specificity and low sensitivity. b) High specificity and high sensitivity. c) Low specificity and high sensitivity. d)_High specificity and low sensitivity.