7 On what part of a microcentrifuge tube should you write a label?

Microbiology for Surgical Technologists (MindTap Course List)
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Author:Margaret Rodriguez, Paul Price
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What does the phrase "pipetting up and down" mean and why is this technique used?
The phrase "pipetting up & down" means to triturate. Th
technique is used to mix (or homogenize) a solutic
7 On what part of a microcentrifuge tube should you write a label?
Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum
efficiency? If not, what order would be most efficient?
9 What does the phrase "spinning down" mean and why is this technique used?
The word 'spinning down" means, To diminish in energy i to slow down o
out; to be gradually ended or concelled. It is used to reduce its spin
speed from that required for reading and writing.
10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime-
The comb is in the center of the gel Since the dyes used have
positive or negative charges and can therefore migrate in differen
Transcribed Image Text:What does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differen
-re there any
, to what did
g. The density
00 μL in 1 mL,
water weigh?
st 0.01 g) of
acting the
of the tube
the space
a permanent marker, such as a Sharpie, that will not
erase or bleed if it gets wet.
2 Using one hand (as described in Procedure 3, Step 7),
open the lids of all the microcentrifuge tubes so they are
ready to receive the solutions. Before you begin measur-
ing, think about what will be the most efficient way of
dispensing the amount.
Important: Always use a new tip for each different liquid
that is being transferred. A new tip should also be used if it
touches a liquid other than the one that is being
transferred.
You may want to first fill all of the empty tubes that
have the same measure of liquid so you do not have to
change the setting too often. Sometimes it matters which
ingredient is added first, as is the case when diluting
acids and bases. In this procedure, the order does not
CHAPTER 14 | Molecular Biology
149
Transcribed Image Text:-re there any , to what did g. The density 00 μL in 1 mL, water weigh? st 0.01 g) of acting the of the tube the space a permanent marker, such as a Sharpie, that will not erase or bleed if it gets wet. 2 Using one hand (as described in Procedure 3, Step 7), open the lids of all the microcentrifuge tubes so they are ready to receive the solutions. Before you begin measur- ing, think about what will be the most efficient way of dispensing the amount. Important: Always use a new tip for each different liquid that is being transferred. A new tip should also be used if it touches a liquid other than the one that is being transferred. You may want to first fill all of the empty tubes that have the same measure of liquid so you do not have to change the setting too often. Sometimes it matters which ingredient is added first, as is the case when diluting acids and bases. In this procedure, the order does not CHAPTER 14 | Molecular Biology 149
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