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A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila *² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY

A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila *² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY

Biology 2e
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter10: Cell Reproduction
Section: Chapter Questions
Problem 19RQ: Which protein is a positive regulator that phosphorylates other proteins when activated? p53...
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Fig. 1. AKAP11 and RIα are degraded by autophagy. (A) Brain lysates of Atg7F/F (Control) and Atg7F/F-SynCre (cKO) were subjected to immunoblotting
analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student’s t tests were used, and values are presented as mean ± SEM
(n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F/F (Control) and Atg14F/F-SynCre (cKO) were subjected to
immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student’s t tests were used, and values are presented as mean ± SEM (n = 4 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using
EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E
was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype,
and values are presented as mean ± SEM (n = 3). *P < 0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the
indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was
obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and
values are presented as mean ± SEM (n = 4). #
P < 0.05; **P < 0.01; ***P < 0.001. 

explain each diagram from A-H please!

A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila *² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY
Transcribed Image Text:A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila *² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY
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