A purified protein is in a 100. mM HEPES buffer pH 7.3 with 500. mM sodium chloride (NaCl). A 25.0 mL sample of the purified protein in a tube made of dialysis membrane is dialyzed against a 1.50 L solution of 25 mM HEPES buffer pH 7.3 without any sodium chloride. The dialysis membrane allows for small molecules and ions (such as sodium ion, chloride ion , and HEPES) to diffuse, but not the protein of interest. What is the final concentration of the HEPES AND sodium Chloride after the dialysis reaches equilibrium? Answer choices: a. 1.64 mM HEPES and 8.20 mM NaCl b. 26.2 mM HEPES and 32.8 mM NaCl c. 26.2 mM HEPES and 8.20 mM NaCl d. 1.64 mM HEPES and 0.00 mM NaCl e. 62.5 mM HEPES and 32.8 mM NaCl
Ionic Equilibrium
Chemical equilibrium and ionic equilibrium are two major concepts in chemistry. Ionic equilibrium deals with the equilibrium involved in an ionization process while chemical equilibrium deals with the equilibrium during a chemical change. Ionic equilibrium is established between the ions and unionized species in a system. Understanding the concept of ionic equilibrium is very important to answer the questions related to certain chemical reactions in chemistry.
Arrhenius Acid
Arrhenius acid act as a good electrolyte as it dissociates to its respective ions in the aqueous solutions. Keeping it similar to the general acid properties, Arrhenius acid also neutralizes bases and turns litmus paper into red.
Bronsted Lowry Base In Inorganic Chemistry
Bronsted-Lowry base in inorganic chemistry is any chemical substance that can accept a proton from the other chemical substance it is reacting with.
A purified protein is in a 100. mM HEPES buffer pH 7.3 with 500. mM sodium chloride (NaCl). A 25.0 mL sample of the purified protein in a tube made of dialysis membrane is dialyzed against a 1.50 L solution of 25 mM HEPES buffer pH 7.3 without any sodium chloride. The dialysis membrane allows for small molecules and ions (such as sodium ion, chloride ion , and HEPES) to diffuse, but not the protein of interest. What is the final concentration of the HEPES AND sodium Chloride after the dialysis reaches equilibrium?
Answer choices:
a. 1.64 mM HEPES and 8.20 mM NaCl
b. 26.2 mM HEPES and 32.8 mM NaCl
c. 26.2 mM HEPES and 8.20 mM NaCl
d. 1.64 mM HEPES and 0.00 mM NaCl
e. 62.5 mM HEPES and 32.8 mM NaCl
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