A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 ml of Solution 1 into a 25 ml volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was

A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 ml of Solution 1 into a 25 ml volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter1: Introduction

Section: Chapter Questions

Problem 1.9QAP

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Differentiate between qualitative analysis and quantitive analysis?
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A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
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