A target gene for producing human insulin must be inserted into a plasmid before the transformation process. Why the plasmid is important in this process? I The target gene lacks the ability to replicate itself. II Screening process will be difficult without cloning vector. III The host cell is able to accept the recombinant plasmid but not the non- recombinant plasmid. O I and II only I and III only O Il and III only O I, Il and III

A target gene for producing human insulin must be inserted into a plasmid before the transformation process. Why the plasmid is important in this process? I The target gene lacks the ability to replicate itself. II Screening process will be difficult without cloning vector. III The host cell is able to accept the recombinant plasmid but not the non- recombinant plasmid. O I and II only I and III only O Il and III only O I, Il and III

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter15: Dna Technology And Genomics

Section: Chapter Questions

Problem 10TYU: DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c)...

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E. coli cells usually have only one copy of the F plasmidper cell. You have isolated a cell in which a mutationincreases the copy number of F to three to four per cell.How could you distinguish between the possibility thatthe copy number change was due to a mutation in theF plasmid versus a mutation in a chromosomal gene?
One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N culture and then resuspend them in buffer P1. What's the point of pelleting the cells just to resuspend them again? To concentrate the cells P1 is a buffer, preventing small changes in pH O This step is not absolutely necessary P1 begins the lysis process D Question 2 What would happen if we didn't centrifuge the tube containing lysed cells after adding neutralization buffer and instead just added right to the column? O We could fihish the prep but we would have protein/RNA contaminants at the end O The precipitate is less dense than water so it shouldn't get in the way since it floats on top O The precipitate would obstruct the column and our prep is ruined O Our yield would be reduced, but we'd get something
What type of molecule or cell is PGLO? C Origin region Arabinose operon arac ori Ndel Promoter PaaD PGLO (5,400bp) Ndel Ampicillin Resistance Gene Amp Hindll Ndel Green Fluorescent Protein (GEP) Gene Hindll 'ECORI GraphiceESchmid/2003 D O virus O RNA plasmid molecule O DNA plasmid moelcule O none of the choices are correct
In the genotype presented (genomic plus plasmid genes), which of the following statements Is phenotype? genome: IS P O`z+Y° plasmid: I* Pt otzY+ no production of either B-gal and permease O B-gal and permease induced when lactose is present no B-gal is produced and expression of permease when lactose is present constitutive expression of B-gal and permease constitutive expression of B-gal and no expression of permease
THIS EXPERIMENT to transform competent cells and p LBAX plates, you see only white colonies on both QCt. What does this suggest about your experiment? X-gal wasn't added to the LB plates. O The Dpn1 digestion didn't work. The PWS4.5 plasmid was contaminated with Lac Z+ genes.
A host cell has successfully taken up a recombinant pUC18 plasmid but the plasmid bears mutated origin of replication. Which of the following will happen to that host cell following amplification process on a media containing ampicillin and X-gal? * I It fails to form a colony on the media It survives in the media It would not be able to digest X-gal II III I and II only O I and III only Il and III only I, Il and III
Alu 1 EcoRI ori pKY Bgl I Tet: Tetracycline resistance gene A. What would be the donor organism to be used in this cloning? B. How would you make sure you only get your gene of interest (EGF) from donor but nothing else? C. How do you put EGF gene into pKY plasmid? What is the best RE to use when inserting the gene into pKY plasmid? What happens if you use the enzyme Bgl I to insert the gene of interest? How does it affect your cloning result? D. How do you make sure you only grow E.coli that have the gene of interest?
You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony.When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
Each gene has its own promoter. For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and which is/are inducible and constitutive?
During which step in the transformation protocol does the plasmid DNA move into the cell? Heat Shock Grow in LB media at 37°C Plate on media with Ampicillin O Incubate the cells on ice with CaCl2 in the absence of plasmid. fg f1o 19 %24 & 5 6. 7 8. 4 5 R Y U P F G J 2 K 3] vの

Sal1 Xho1 Insert pUC cloning vector MCS Insert Digested with Sal1 on the left and Xho1 on the right Sal1 Ori Vector Digested with Sal1 Xho1: СТСGAG Sal1: GTCGAC RE cut site Part B) After 8 h of sleep, you realize your mistake above (Question 3 Part A), and you successfully transformed your plasmid and obtained a subset of white colonies and blue colonies on the plate. Question: You conclude that all the blue colonies contain a plasmid O With the insert in the LacZ screening marker With the insert in the Antibiotic selectable marker O Without an insert in the LacZ screening marker O None of the above + Lacz alpha AmpR
Choose the false statement. O Genetic engineering includes recombinant DNA techniques whereby DNA is hydrolyzed at specific sequences, pasted back together with ligase, and then inserted into cells of interest. O GMOS are organisms that, if eaten as a nutrient source, can genetically modify our DNA. OBacterial cells that have been transformed with the engineered plasmid can be selected for by utilizing antibiotics in the media and adding a drug resistance gene to the plasmid.