A TLC of spinach leaves experiment is performed. The solvent front directly above the spot traveled 12.45 mm. The spot traveled 6.28 mm. What is the Rfvalue for the green pigment of the spot? Select one: 6.17 1.98 0.50 6.28 O O
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- After performing a TLC experiment, a researcher determines the Rf value of a component to be 2. He also notes that the solvent travelled a distance of 4cm on the plate. What can you conclude about this experiment? C) The solvent is not volatile D) The reported data does not seem valid B) The component travelled a distance of 8cm OA) There are multiple components in the mixtureWhat is the Rf value given the following information: Height of Chromatography Paper: 5.0 cm Distance from Bottom of Paper to Origin: 1.5 cm Distance from Origin to Spot: 1.6 cm Distance from Origin to Solvent Front: 2.9 cm Your Answer: AnswerA student performs this and records the following data from the complted chromatogram. the solvent has moved 7.30 cm Component A produces a blue spot that has moved 5.80 cm. Component B produces a pink spot that has moved 2.45 cm. Component C produces a tan spot that has moved 4.10cm. Calculate the Rf values for component.
- The Rf of four spots in a normal phase paper chromatography is given. Determine which of these is the most polar. Rf= 0.40Rf= 0.25Rf= 0.21Rf= 0.34If the stationary phase and the compound being tested are both polar, will the retention factor (Rf) likely be smaller or larger than if a nonpolar compound was tested? Why? 1) The retention factor will likely be smaller as it has a smaller interaction with the stationary phase O 2) The retention factor will likely be greater as it has a smaller interaction with the stationary phase O3) The retention factor will likely be smaller as it has a greater interaction with the stationary phase 4) The retention factor will likely be greater as it has a greater interaction with the stationary phaseWhat are the advantages of using the KBr in IR spectroscopic sample preparation? Please shortly write at your own words. (4-5 lines maximum ).
- calculate Rfs for each spot on your chromatogramIn TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so slowly?A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
- Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot moved 9.4 cm from the origin. Type answer: 1.5411. Looking at the structures of the 4 different drugs below, what do they all have in common, explain. HO aspirin caffeine НО 12. When recording the Rf of a compound, what factors are important to know about the TLC run? What factors can change the Rf?5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.
