Please correct answer and don't use hend raiting After completing affinity chromatography run,need to do an 9:1 dilution with PBS, 0.001% W/Vpoloxamer 188, pH 7.4 with the elution pool. Ifthe eluate is 3.5column volume (CV) how muchPBS do I need to make with 1.5% extra ?

To calculate the amount of PBS needed to dilute the eluate from an affinity chromatography run, you…

Answered: After completing affinity…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.13QAP

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After completing affinity chromatography run, need to do an 9:1 dilution with PBS, 0.001% W/V poloxamer 188, pH 7.4 with the elution pool. If the eluate is 3.5column volume (CV) how much PBS do I need to make with 1.5% extra ?