An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc should be 1x and answer shoud be in ul
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- An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: м 1 M 2 3 4 ng/0.5 µg bp 20 5000 20 4000 40 3000 20 2000 20 1000 60 500 20 100 Determine the amount of the total DNA ladder added in the gel in terms of ugWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: M 2 3 4 ng/0.5 µg bp 5000 20 4000 40 3000 20 2000 20 1000 60 500 100 Compare the bands in the Lanes 1,2,3,4 with the nearest DNA standard by determining the DNA standard which is nearest in size to the samples in Lanes 1,2,3,4 DNA standard (choices: 100, 500, 1000, 2000, 3000, 4000, or 5000 bp) DNA sample Lane 1 bottom band Lane 1 top band Lane 2 band Lane 3 band Lane 4 band 20 20
- Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilutionFor a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. Purity of DNA - OD 260/280 %3D Concentration of DNA = Abs x 50 µg/ml x dilution factor (100) 0.125 x 50 µg/ml x 100 = Edit View Insert Format Tools Table 12pt v Paragraph v BIUA 2 T'vComplete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Total μL µL/Rxn (total per tube) Final Number of Stock Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 µM MgCl2 Forward 24 mM 2.5 mM 2 μΜ 0.1 μΜ primer Reverse 2 μΜ 0.1 μΜ primer Taq polymerase DNA 5 U/uL 0.03 U/ µL 1 μL 60 μL Total volume of the entire reaction (µL)
- researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another lane. The gel is 0.5% agarose, and it is run for 45 minutes time at 200 V. When he checked the gel, he saw three bands in the Hind III lane and no other bands on the gel. What can be the reason behind this. What additional information we may need to be sure about the reason.If the DNA was replicated using the dispersive model, what would you have expected to observe in the generation 2 sample? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Question 6 Homework Answered 6.0 g 3 distinct bands (one 15N, one 14/15N, and one 14N) 2 distinct bands (one 15N and one 14N) 2 distinct bands (one 15N and one 14/15 hybrid) Only one distinct band (15N) f Only one distinct band (14N) Only one distinct band (14/15 hybrid) No bandsThe results of gel electrophoresis of 4 different DNA samples given in the figure. 16 ul was loaded into each well (6 μL diluted DNA* + 8 μL water + 2 μL sample buffer). The ladder is in lane 5, with the size and amount (ng) in each band indicated. *1:5 dilutions of the original DNA sample were made, and the diluted samples were used for AGE. Q. These are the conclusions made about sizes and concentrations of the original (undiluted) DNA samples from lanes 1 and 2. Are these conclusions reasonable? Explain specifically, and if any of the conclusions are not reasonable, explain why not and what should we conclude instead. Sample in lane 1:size is 2.32 kb concentration of original DNA sample is 9.2 ng/ul Sample in lane 2:size is 4.36 kb concentration of original DNA samples is 100 ng/ul Q. The sample in lane 3 was expected to be about 2 kb in size. What is a possible explanation for the results observed?
- Figure 4 shows the results from Nanodrop quantification of DNA sample. Sarmple ID E Coli (K-12) Pedestal 14 Турe DNA 50 00 Conc. 843.6 ng/ul A260 (10 mm path) A280 (10 mm path) 260 / 280 2.11 260 / 230 2.24 O Baseline correcton 340 nm Wavelength (nm) Figure 4 (i) Interpret the Nanodrop result above. (ii) How to improve the quality of the extracted DNA in order to obtain a better result? (ii) Calculate the absorbance of DNA at 260nm. 10mm AbsorbanceCompute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA extraction. a. 10 mM Tris b. 1 mM EDTAFor letter A, pls ILLUSTRATE (create an illustration or drawing) the DILUTION SERIES of the problem just like the sample on the 2nd image. Please read the instructions carefully as I have already posted this twice and the experts just copy-pasted the answers from my first post. I don't want to waste another post question for this one. Again, I NEED AN ILLUSTRATION and not just the computation/explanation through words so I can properly visualize the problem. WILL UPVOTE if I get what I need.