& protein in a solution measured in a 96 well plate You have the following information: volume in well-200 ul., pathlength 6 mm, -43824 M1cm1, Absorbance 020. Choose the most correct answer 0476x 100 M Ob.76 M c76mM Od 7.6
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- The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of five 50.0-mL volumetric flasks. Various volumes of a standard containing 12.2 ppm Cr were added to the flasks, following which the solutions were diluted to volume. Unknown,mLStandard, mLAbsorbancc 10.00.00.201 10.010.0 0.292 10.020.0 0.378 10.030.0 0.467 10.040.0 0.554 (a) Plot the data using a spreadsheet. (b) Determine an equation for the relationship between absorbance and volume of standard. (c) Calculate the statistics for the least-squares relationship in (b). (d) I)ctcrmine the conccnt ration oÍCr in ppm in the sample. (e) Find the standard deviation of the result in (d).You would like to calculate the concentration of a protein in a solution measured in a 96 well plate. You have the following information vo in well = 200 μL. pathlength=6 mm, 25000 M1 cm1, Absorbance-2.40. Choose the most correct answer Selected Answer: d. 0.16 mMIn a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
- You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).Iam stuck on how to start this problem. Youo are presented with a sample of freshly squeezed carrot juice and are asked to determine how much beta-carotene is presented in the juice. A beer's Law plots is constructed from the absorbances at 451 nm for six standard solutions of beta-carotene, with concentrations ranging from 0.75-7.5 µM. Linear regressions data: y=0.1431x-0.0078(R2=0.9998) a)The carrot juice is diluted by one-fourth, and the absorbance at 451 nm of this solutions is measured to be 0.676. Based on this information, what is molor concentration of beta-corotene in the original(undilited) sample of carrot juice? b) If the original sample of carrot juice was 312 mL, calculated the mass(g) of beta-carotene presentedYou obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
- CPU/RAM ProctorU e Proctor e Proctoru o.com/collab/ui/session/join/43918c0bf9cb421ca7b98b0075f5f239 Firefox Ex SPECTROSCOPY 1. If the concentration of solute in a solution is high, the Absorbance will be high or low and why? 2. Was the spectroscopy experiment qualitative or quantitative analysis? 3. If we have 12M HCI and we need 3 L of 1M HCI, how much 12 M should we use? 4. What does Molarity mean? 5. When must safety goggles be worn? 6. If you make a mistake recording data, what must you do? spec hw.pptx (2/3) ..% AnYou are studying the absorbance properties of the COVID-19 virus. You notice at (4.9500x10^-1) absorbance, the molar absorptivity of the G variant is (4.7887x10^5) M1 cm in hexane at (5.3700x10^2) nm. The concentration was determined to be (3.60x10^-4) M. What is the pathlength of the cuvette in nanometers (nm)? Please do not include units in the answer and present your answer in scientific notation. Note: Your answer is assumed to be reduced to the highest power possible. Your Answer: x10 Answer10 Wavelength, 1. (nm) E (Red, µ.Mcm- ɛ(Yellow, µMr'cm¬1) Absorbance 500 0.0369 0.000549 0.405 430 0.00506 0.0167 0.474 An orange colored solution is prepared by mixing a red dye solution with a yellow dye solution and the absorbance was measured at the analytical wavelengths of 430 nm and 500 nm. The table provides the molar absorptivity for the red and yellow dye at each analytical wavelength. Calculate the concentration (in u.M) of the red and yellow dye in the mixture. [red] = [yellow] =
- part A:> to Calculate Concentzahion, use M,V, = M2V2 AB1 - 0.025 M X 2.5 ml = ax 5 ml %3D 0.0125 M 0.01 M x 2. S ml= 5°-]x Ŝ ml [s2] = 0.008 M 0. 002 M 11 2 a - X = 0. 01 M Sohtion a 9- X timels) 9-X ABI 0-0125 0-0025 0.01 0. 223 84.8 0.016구 0.0016구 0.015 0.10 구 니1.2 AB 2 0.0 S16 19.5 O.02 0. 00 1 0. 이19 AB 3 0. 0164 0.0816 28.5 0.이구8 0. 0014 AB 니 0. 0022 0.011 구 0.172 60 AB § 0.이139 0.270 95.2 O. ol14 0.0027 0.008구 A8 6 0. 389 14 5.5 0.0096 0.0031 0.0065 AB구In a Bradford assay, 17 pl of a protein isolate sample was diluted by adding 24 pl of water prior to the addition 2.0 mL Bradford reagent, Calculate the protein concentration (in mg/ml) of the original protein isolate sample whose absorbance reading at 595 nm is 0,384 after dilution The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 3 6 conc of BSA Kug/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xox (three decimal places) Round off only in the final answer. Do not round off in the middle of the calculationThe absorbance values at 250nm of 5 standard solutions, and sample solution of a drug are given below: Conc. (ug/ml) A 250 nm10 0.16820. 0.32930 0.50840. 0.66050 0.846Sample. 0.661Calculate the concentration of the sample