A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. a. What is the expected result if the protein stays permanently in the nucleus? b. What is the expected result if it is a shuttling protein? Explain your answer.

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.