At pH 12.48, arginine (pK's 1.82, 8.99, 12.48) would be charged as follows: O 1) -1 alpha-carboxyl, 0 alpha-amino, +0.5 guanidino, -0.5 net charge O 2) 0 alpha-carboxyl, 0 alpha-amino, +1 guanidino, +1 net charge 3) +1 alpha-carboxyl, -1 alpha-amino, -1 guanidino, -1 net charge 4) +1 alpha-carboxyl, 0 alpha-amino, -1 guanidino, 0 net charge
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- A mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2. v First 1. Aspartic Acid v Second 2. Histidine v Third 3. Lysine 4. Phenylalanine v Fourth 5. Threonine v Fifth 6. No separationWhat is the charge on the following peptide at standard biochemical pH? S-Y-D-F-K-I-V-F-L-L +2 -1 0 0 0-2 O +1draw the primary structure of the polypeptide at physiological pH range of 7.35-7.45 using condensed structures 3-letter abbreviations with a hyphen representing peptide bond give the name of the peptide, determine the net charge, and calculate the isoelectric point KRINPLA
- pKal 2.19 pKa2 9.67 pKa3 4.25 Amino acid Glutamic acid Threonine |Isoleucine Arginine 2.63 2.36 2.17 9.10 9.68 9.04 12.48 Draw the predominant ion of glutamic acid at its pl. Given the R group of glutamic acid is CH,CH,COOH. (ii) Draw a tripeptide of Gly-Ser-Val. Also indicate in the structure the peptide bond, N-terminus and C-terminus. (R groups for Gly, Ser, and Val is H, CH,OH and CHCH;CH; respectively).14 12 10 pH 8 6 4 0 Titration curve for an unknown amino acid pk₁=1.8 pK₂=6.0 pK, 9.2 Volume (mL) of 1 M NaOH added A student titrated a 250 mL solution of an unknown amino acid with 1.0 M sodium hydroxide and drew the below titration curve. From this titration curve they found that the amino acid had three functional groups with the pKa of the functional groups displayed on the graph. To do this titration they added in a total of 75 mL of 1.0 M sodium hydroxide. They also calculated that they added a total of 15 mL of sodium hydroxide to get to pK₁ (indicated by arrow) and a total of 37 mL of sodium hydroxide to get to pK₂(indicated by arrow). What was the initial concentration of the amino acid solution in milimolar (mM)? Provide the answer with NO decimal places. You do not have to indicate the unit in the answer field!At pH 7.0, in what order would the following three peptides (described by their amino acid composition) be eluted from a column filled with a cationexchange polymer?Peptide A: Ala 10%, Glu 5%, Ser 5%, Leu 10%, Arg 10%, His 5%, Ile 10%, Phe 5%, Tyr 5%, Lys 10%, Gly 10%, Pro 5%, and Trp 10%.Peptide B: Ala 5%, Val 5%, Gly 10%, Asp 5%, Leu 5%, Arg 5%, Ile 5%, Phe 5%, Tyr 5%, Lys 5%, Trp 5%, Ser 5%, Thr 5%, Glu 5%, Asn 5%, Pro 10%, Met 5%, and Cys 5%.Peptide C: Ala 10%, Glu 10%, Gly 5%, Leu 5%, Asp 10%, Arg 5%, Met 5%, Cys 5%, Tyr 5%, Phe 5%, His 5%, Val 5%, Pro 5%, Thr 5%, Ser 5%, Asn 5%, and Gln 5%
- You are given 0.5 M solution of the amino acid Arginine. pK1 (α-carboxyl group) = 2.17 pK2 (α- amino group) = 9.03 and pK3 (guanidine group) = 12.48 Determine the pH of the solution if you add 15 mL 0.25 M HCl to 10 mL of the 0.5 M Arginine. Assume that Arginine is in isotonic state. Show all calculationsYou are given 0.5 M solution of the amino acid Arginine. pK1 (α-carboxyl group) = 2.17 pK2 (α- amino group) = 9.03 and pK3 (guanidine group) = 12.48 Determine the pH of the solution if you add 15 mL 0.25 M HCl to 10 mL of the 0.5 M Arginine. Assume that Arginine is in isotonic state. Show all calculations b. Draw the structure of the amino acid at the pH determined in question 1draw the primary structure of the polypeptide at physiological pH range of 7.35-7.45 using condensed structures 3-letter abbreviations with a hyphen representing peptide bond give the name of the peptide, determine the net charge, and calculate the isoelectric point DFACKT
- A mixture of Asparagine (pl 5.41), Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2: v First 1. Asparagine v Second 2. Aspartic Acid 3. Histidine v Third 4. Lysine v Fourth 5. Threonine v Fifth 6. No separationCHEM 153 Organic/Biochemistry Exercise 2: Proteins Names: Answer as directed. 1. Assign IUPAC names to each of the following small peptides. a. Gly-Ala-Leu b. Gly-Tyr-Ser-Ser C. Ex cu & H₂N-CH-C-N-CH₂-C-N-CH-C-0- H (CH₂)4 NH₂+ + CH₂ ||| HDetermine the net charge (to the nearest integer) on the following peptide at pH 5 AND pH 12. Estimate the isoelectric point (pl) for this peptide: H2N-Leu-Gly-Lys-Glu-COOH Assume the pK,s for functional groups are: alpha-amino 6. alpha-carboxy sidechain-amino (Lys) 10.5 sidechain-carboxy (Glu) 4.2 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =6.6 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =3.1 O Net charge at plH 5 and 12 is +2 and -1, respectively, pl =7.5 O Nct charge at pH 5 and 12 is +1 and -1. respectively,. pl -4.5 2.