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- state at low partial pressure of oxygen to release more oxyge 2. Hemoglobin is able to reversibly bind oxygen (O₂) due to (a) cooperativity (b) proximal histidine (c) distal histidine (d) ferrous ionMatch the peak chromatogram and the possible compound based on the information given (example: Peak A – Compound A). All peaks originated from one HPLC injection. 0.14 Peak 1 Peak 2 0.12- Peak 3 0.10- 1564 0.08- 1730 1345 Peak 4 0.06- Peak A 1311 0.04 Рeak B 567 1211 0.02- 0.00- 0.00 0.50 1.00 1.50 2.00 3.00 3.50 4.00 5.00 250 min 4.50 (a) Mode: Normal phase HPLC Peaks: 1, 2, 3 and 4 Compounds mix: Aspartame; Benzoic Acid; Caffeine; Sorbic Acid NH 2 H ОН ОН N' Benzoic acid Caffeine Sorbic Acid AspartameWhat are the main four differences between Nmap and Hping3 with examples?
- Drugs called COX-2 inhibitors (e.g., Vioxx, Bextra, andCelebrex) were thought to relieve the pain and inflammation ofosteoarthritis without the stomach bleeding and ulcers non-steroidal anti-inflammatory drugs (NSAIDs) cause. In a 12-month trial, Vioxx caused fewer gastrointestinal side effectsthan the NSAID ibuprofen. However, a study of the recurrenceof colon polyps after three years of Vioxx found an increasedrisk for heart attack and stroke beginning after 18 months oftreatment. As a result, Vioxx was withdrawn from the market,and an FDA panel concluded that COX-2 inhibitors as a classhave increased cardiovascular risk that varies by drug anddose. The FDA then caused the withdrawal of Bextra and re-quired a warning label on Celebrex. Based on this information,list (a) an observation, (b) a hypothesis, (c) an experiment, and(d) a theory.EDTA is a hexaprotic system with the pK, values: "ooc-CH2 H,C-coo pKal = 0.00, pK2 = 1.50, pK = 2.00, pK4 = 2.69, :N-CH-CH-N: pKas = 6.13, and pK6 = 10.37. %3D "ooc-CH2 HC-coo The distribution of the various protonated forms of EDTA EDTA“ (or Y-) will therefore vary with pH. For cquilibrium calculations involving metal complexes with EDTA, it is convenient to calculate the fraction of EDTA that is in the completely unprotonated form, Y. This fraction is designated aya. Calculate ay at two pH values. 6.33 X10-13 pH = 3.15 ay = Incorrect 0.1327 pH = 10.55 dy = %3D Incorrect1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?
- What kind of chromatography would you for purification of full length antibody ?+Protein Mr pl А 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 3 8.4 C 7.2 9 E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute First LastAvapH dT T(V, – Vi) dP AvapH _ AvapH _ AvapH TV -P RT2 RT AvapH For a change of state, ApapS Tvap P Deduce the relationship between In- and AvapH, AvapS, and T.
- The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular mass ( M, ). A newly purified protein (X) run on the same gel showed a single band that had moved a distance of 55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm? Round off your answer to the nearest whole number. Protein M. Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 Glyceraldehyde-3- phosphate dehydrogenase carbonic anhydrase 36 000 38.0 29 000 50.0 Trypsinogen 24 000 54.0 Soyabean trypsin inhibitor 20 100 61.0 Myoglobin 17 800 69.0 Lysozyme 14 300 79.0 Cytochrome c 12 400 86.52) Part of the 'H NMR spectrum for a dipeptide recorded in D₂O is shown below. The signal for the CH 3 group is not shown. (i) Explain what has happened to the H-atoms attached to the N atoms. (ii) Interpret the spectrum Note the signals for HA and HA are subject to a second order effect. 'N- HAHA' 40 Hz H 0.1 ppm L-Ala-GlyIs TMA a volatile compound or a biogenic amine? (explain further) Is the measurement of TMA a reliable index of spoilage? Why and why not? (explain further)