(b) Protein concentration can also be determined by UV spectrophotometry. (i) Describe the principle of this method List three advantages of this method Explain why nucleic acids can interfere with this assay (ii) (iii)

(b) Protein concentration can also be determined by UV spectrophotometry. (i) Describe the principle of this method List three advantages of this method Explain why nucleic acids can interfere with this assay (ii) (iii)

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter10: Fuels, Organic Chemicals, And Polymers

Section: Chapter Questions

Problem 42QRT

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

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(b) Protein concentration can also be determined by UV spectrophotometry.
Describe the principle of this method
List three advantages of this method
(iii) Explain why nucleic acids can interfere with this assay

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