Bacteria count Shown is a typical graph of the number N of bacteria grown in a batch culture as a function of time 1. Describe what you think is happening during each of the four phases. NA
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- A bacterial strain has a generation time of 30 min, how long would it take an exponentially growing culture to increase from a titre of 5 x 103 cells per ml to 4 x 109 cells per ml? How many generations would have elapsed? Show all of your calculations. G = t/n to calculate generation time (i.e. for population to double) During the growth of the culture, periodic observations of the cells under the microscope have revealed the occurrence of typical rod-shaped cells. However, after further incubation, they appeared to change their morphology to form small oval or spherical-shaped structures. What do these structures likely represent? And what are the likely factors contributing to this observation? Explain what growth phase this culture will be in.Bacteria can increase in their number by binary fission into two daughter cells. The rate of exponential growth of a bacterial culture is expressed as generation time, how can the growth curve effect on generation time bacterial؟Describe how to most easily calculate the generation time for a population of bacteria in a batch culture in a closed system. Also explain why the calculation is made in a special phase of the growth curve
- Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.Bacteria growth and multiplication play a role in infectious diseases : Bacteria can increase in their number by binary fission into two daughter cells. The rate of exponential growth of a bacterial culture is expressed as generation time, how can the growth curve effect on generation time bacterial?A species of bacteria has a doubling time of 24 hours. After incubating the initial population for one week, the final population was 6,400 cells. What was the initial population of that bacteria? Show all work. Equations: Nt = No x 2n Final concentration = (initial concentration)/(total dilution factor) Total dilution factor = (individual dilution factor)# of serial dilutions
- Devise a scheme to prepare a 10-9 dilution spread plate, using the least number of dilution tubes. Note: It is not practical to pipette any volume less than 0.1 ml and your equipment is similar to that in the Micro-lab, you only have 10 ml dilution tubes and a supply of sterile water Describe the growth patterns of each bacterial isolate that you observed in the nutrient agar deeps (shake cultures) and state what this says about their metabolism. Give definitions of these bacterial growth in relation to O2 E. coli: turbid growth throughout Micrococcus luteus:Growth on the top Clostridium sporogenes: growth on the bottomIn growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees celsius and under 37 degrees celsius, the number of colonies after doubling time has decreased? provide reliable sources (links).There are 10 mL of a culture of a bacteria at 104 cells/mL into 990 mL of the same broth at the same temperature (37° C) It is 8 p.m. the night before your lab which starts at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before your lab? Set up the equation correctly, solve for t, and tell me what time the culture will reach Stationary Phase. t /g = (Log Nt – Log N0) /0.301
- Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.A bacterial culture contains 500 cells/mL in the exponential growth phase at 8 AM. If you consider a generation time 20 minutes, what will be the total number of cells/mL after three hours (i.e. at 11 AM)?A bacteria colony's radius is measured as a function of time. t r(t) 0 1.01 0.1 1.031 Estimate the rate of growth Ar/At at t = 0.1 Give two digits past the decimal point. 0.2 1.052 0.3 1.074 0.4 1.096