Based on your observations of microorganisms in their living state, which between the wet mount method and the hanging drop method is better? Why?
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Based on your observations of microorganisms in their living state, which between the wet mount method and the hanging drop method is better? Why?
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- What are the difference in the movement of microorganisms under wet mount from hanging drop technique? Provide a descriptive explanation.Could you explain the difference ( and possibly show the difference) between a growth on Agar slants and Growth in broths? Next, could you describe what E. Coli, M. Luteus, and an uninoculated growth should look like on both agar slants and broths? Much thanks! I am a bit confusedRegarding turbidity, which of the following statements is true? Group of answer choices Decreased bacterial concentration results in a turbid specimen due to increased light transmission Increased bacterial concentration results in a turbid specimen due to decreased light transmission. Increased bacterial concentration results in a clear specimen due to decreased light transmission.
- As part of an experiment where absorbance values are measured using a spectrophotometer, you are taking readings of your sample every 20 minutes. The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature. Things start out fine, with the expected results — as time goes by at the correct incubation temperature, absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms. But roughly 2 hours into the process, you notice that the absorbance levels flatten out, and then start to decrease unexpectedly. What is most likely taking place in your experiment?A study of microbial pathogen transport in an aqueous environment is to be studied. The target microorganism is Escheria Coli with a characteristic length of 25 micrometers. The pathogen moves at a rate of 2.5 micrometer/second. What model fluid should be used? Why?What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?
- Suppose you inoculated two 0.4% semi-solid agar deeps: one deep was inoculated with a motile organism and the other deep with a non-motile organism. You (in a stroke of genius) found a way to quantitatively analyze the deeps using a spectrophotometer. Which deep would have the highest absorbance and why?What is the BEST glassware that could be used to hold the agar powder while weighing? After 24 hours, growth can be seen from your plates. If you want to put dyes on your sample, what rack is used?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- List 5 physical methods of controlling microbial growth, and give an example of each. Why is moist heat much more effective than dry air?When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibioticpropose a hypothesis regarding the organisms (staphylococcus, escherichia, pseudomonas, and bacillus) that will be resistant to the most disinfectants, and why? if the hypothesis is supported, what specific experimental results will be observed? what would be the independent and dependent variable? zone size, disinfectant type, colony count