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- If gene transcription is inhibited after fertilization, development past the two-cell stagedoes not take place in the mouse. However, in frog embryos, this treatment does notdisrupt development until late cleavage. Explain this difference.A C. elegans (nematode) gene called par-1 helps todetermine the AP axis of the animal early in development. Scientists determined that par-1 is pleiotropic—it also has a later function in forming the vulva of theadult animal. How could researchers circumvent thelethality of par-1− mutants to observe the later function of the par-1 gene? (Hint: C. elegans larvae caneat bacteria expressing RNAi for any gene.)A classical experiment studying the fate determination of stem cells in the developing embryo uses the transplantation of somites from one organism to another. In such an experiment, a scientist transplanted somites 20, 21, and 22 from the right side of the neural tube from five-day-old developing quail embryos into chick embryos. The transplantation was performed in two orientations so that the order of the somites was sometimes reversed. Use the passage to answer the question. Central self-tolerance in the immune system arises when maturing T cells in the thymus undergo apoptosis when they bind to self-antigens. Based on this information, what would MOST likely occur as the chick immune system develops? A. T cells would recognize transplanted quail somites as foreign and rapidly divide. B. T cells would recognize transplanted quail somites as foreign and undergo apoptosis. C. T cells would recognize transplanted quail somites as self and rapidly divide.…
- A classical experiment studying the fate determination of stem cells in the developing embryo uses the transplantation of somites from one organism to another. In such an experiment, a scientist transplanted somites 20, 21, and 22 from the right side of the neural tube from five-day-old developing quail embryos into chick embryos. The transplantation was performed in two orientations so that the order of the somites was sometimes reversed. Use the passage to answer the question. Suppose that somite stem cells commit to their cell fates by three days post fertilization. Which result would be MOST likely in the chick embryos receiving somites transplanted in the reverse order? A. The organs arising from quail somites develop in a normal order because local signaling molecules appropriately pattern the developing organs. B. The organs arising from quail somites develop in a normal order because transcriptional programs activated in the nucleus cannot be reversed.…A classical experiment studying the fate determination of stem cells in the developing embryo uses the transplantation of somites from one organism to another. In such an experiment, a scientist transplanted somites 20, 21, and 22 from the right side of the neural tube from five-day-old developing quail embryos into chick embryos. The transplantation was performed in two orientations so that the order of the somites was sometimes reversed. Use the passage to answer the question. Observations of the developing chick embryo reveal that skeletal muscles develop from the transplanted somites even though motor neurons innervating those muscles arise from chick neural crest cells. Based on these observations, what is MOST likely true? A. Neural crest cells differentiate into somites. B. Neural crest cells fuse with developing somite cells. C. Neural crest cells develop from migrating somite cells. D. Neural crest cells send projections into developing…A classical experiment studying the fate determination of stem cells in the developing embryo uses the transplantation of somites from one organism to another. In such an experiment, a scientist transplanted somites 20, 21, and 22 from the right side of the neural tube from five-day-old developing quail embryos into chick embryos. The transplantation was performed in two orientations so that the order of the somites was sometimes reversed. Use the passage to answer the question. As the somite cells develop into muscles, regular depolarizations in the muscles are required for the brain to properly form circuits with developing organs. Based on this observation, which prediction is MOST likely true? A. The muscles release neurotransmitters to stimulate the innervating neurons. B. The innervating neurons release neurotransmitters to stimulate the muscles. C. The muscles release hormones to stimulate the innervating neurons. D. The innervating neurons…
- “In the early Drosophila embryo, the establishment of different body regionor tissues along, on the one hand, the anteroposterior axis and, on theother, the dorsoventral axis are initiated by clues provided by the egg’smother; however the nature of those signals and the way in which theyoperate is completely different”. Discuss whether this statement is correct.What statement best describes the difference between "fate maps" and "specification"? If cells are transplanted from their normal region in an embryo to a different region in a recipient embryo, such cells will alter their fate, but not their specification. Cell fate map describes the allocation of cells to the germ layers, ectoderm, mesoderm, or endoderm, whereas specification describes the exact tissues that each cell will ultimately become. The fate map of an embryo does not change during development -- the fate map of an egg is the same as the fate map of a late blastula -- whereas the specification map of an embryo changes continually as the embryo's development proceeds. The fate map of a cell is determined by labelling that cell and following it during normal development, whereas the specification state of a cell is determined by culturing a cell in an artificial medium and observing what tissues form from it.A research paper published in the summer of 2012presented a method to obtain the whole-genome sequence of a fetus without any invasive procedure suchas amniocentesis that could on rare occasions causemiscarriage. This new technique is based on the factthat some fetal cells leak into the mother’s bloodstream and then break down, releasing their DNA.Assume that exactly 10% of the DNA fragments inthe mother’s blood serum come from the fetus, whilethe remaining 90% of the DNA fragments in the serum come from the mother’s genome.The investigators collected cell-free DNA from apregnant woman’s bloodstream and subjected it to anadvanced high-throughput sequencing method. Thetable at the end of this problem looks at seven unlinked loci; the number of reads of particular alleles(identified by Greek letters) are shown. You shouldassume for the sake of simplicity that all numericaldifferences are statistically significant (even thoughactual data are never this clean).a. Determine whether each…
- Carefully distinguish between the terms differentiation and determination. Which phenomenon occurs initially during development?Gradients of morphogens can be preestablished in the oocyte. Also, later in development, morphogens can be secreted from cells. How are these two processes similar and different?Discuss the role of homeotic genes in development. Explain what happens to the phenotype of a fruit fly when a gain-of-function mutation in a homeotic gene causes the protein to be expressed in an abnormal region of the embryo. What are the consequences of a loss-of-function mutation in such a gene?