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- 88 Exercise 7: Selective and Differential Growth Medium and Tests Questions 1. Draw your expected results if you were to determine that your bacteria is an obligate anaerobe, an obligate aerobe, a facultative anaerobe, an aerotolerant anaerobe or a microaerophile. 2. Where is the oxidized zone in the tube of FTG? 3. Why is one zone pink in the FTG while the other zone is straw colored? 4. What purpose does resazurin play in FTG? eNursing question re_route Please Please make table to differentiate ITP, TTP, HIT, DIC. ?BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K
- How can you make a final dilution of 10-10 using 5 dilution tubes? Outline your steps.Name: Jictorn Zunter Pre-Lab Questions 1. An unknown solution turned a strip of red litmus paper blue. Is this solution acidic or basic? basic 2. Which solution: 0.20 M NaOH or 0.20 M HCI, would you use to titrate an unknown solution that turned blue litmus paper red? 3. A burette had an initial volume of 5.2 mL and a final volume of 32.6 mL, what is the amount of solution from the burette that was used? Show you calculation below. Vz-V,=32.c6-5.ZML =27.4mL 4. 25.0 mL of an unknown acidic solution was titrated with 42.7 mL of a 0.15 M NaOH solution. What is the M of the unknown acid? Show your calculation below. 5. What is the Normality of 0.25 M H2SO4 6. What is the N of 0.45 M Mg(OH)2 7. Using the formulas provided for you in the lab introduction, fill the missing areas in the following table РОН 8.5 pH [H'1 [OH] 3.7 3.2x108 1.7x103 Show 1 row of calculations, below:Questions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?
- LAB QUESTIONS – ASSESSMENT AND CRITICAL THINKING 1. When is a simple negative stain used? 2. Why do microorganisms remain unstained in the simple negative staining procedure? 3. What information can be obtained from a simple positive staining procedure? 4. What is the difference between a simple and differential stain? 5. What is the reagent and purpose for each of the following gram stain steps? a. Primary stain b. Mordant c. Decolorizer d. Counterstain 6. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 7. What part of the bacterial cell is most involved with Gram staining, and why? 8. What cell wall component is responsible for the acid-fast property of mycobacteria? 9. Is a gram stain an adequate substitute for an acid-fast stain? Why or why not? 10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure…Guide Questions: Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used?Sample Station 5 This is an MRVP broth. The reagents A and B for the VP test have been added. A. What observations can you make about this test? B. What interpretation can you make about this organism's ability to ferment glucose?
- Week 10 - Review Sheet Exercise 3 - Staphylococci 1. Differentiate the microscopic morphology of staphylococci and streptococci as seen by Gram stain. 2. What are the two types of staphylococcal coagulase? 3. What is protein A? Describe one method of detecting it. 4. What properties of S. aureus distinguish it from S. epidermidis and S. saprophyticus? 5. Why are staphylococcal infections frequent among hospital patients?14-26-27-30 Baguio ice plant and cold storage samples must be tested with the presence of total bacteria, total coliform, and thermotolerant coliform once a . A biologist can work as a licensed scientist of a newborn screening laboratorywith five months laboratory experience. Removal of cornea from the decedent should be done within 12 hours afterdeath for the sole purpose of transplantation. Before an organ donation is executed, the dead person must be examinedby two physicians neither of whom should be attending physician of the donne.Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7 3. Set up controls of the donor and recipient cultures as follows: • Spread plate 0.1 ml of the donor culture over the surface of each of the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar + Sm; nutrient agar + Rif) • Similarly spread plate 0.1 ml of the recipient culture over the surface of each of the 3 selective media • What is the purpose of this step?