Catalase Yeast Test
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Catalase Yeast Test Questions:
1. Why is it important to graph volumes that did not include the initial volume?
2. How might the temperature of the yeast mixture affect a reaction?
3. What effect would increasing the amount of yeast have on a reaction?
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- in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g Saccharomyces cerevisiae to 3 mL yeast growing medium (56 mM glucose, 20 mM HEPES, pH 6.8). What percent of yeast suspension is left after a 1:10 dilution?250 uL of 1M azide was added to a 25 mL yeast solution. What was the concentration of azide in the yeast culture? (use proper units)Ex. 5-4 Catalase 1) In the Catalase test (5-4), if bacteria was added to hydrogen peroxide on the slide, it might produce a false result. 2) Explain why adding bacteria to the hydrogen peroxide on a slide rather than adding the hydrogen peroxide to the bacteria on the slide might give a false result? 3) Would the false result be a false positive or would the false result be a false negative?
- Description 1. The fractions obtained from differential centrifugation are enriched but not pure. Explain how a greater degree of purification can be achieved using Density-gradient centrifugation and velocity centrifugation. 2. Explain equilibrium centrifugation.answer the following questions 1.What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer? 2.What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle?3.What is the optimum temperature for invertase reactions?4.What is the optimum ph for the invertase reactions?1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test system? list the concentrations of sugars in the media and the possible results due to fermentationMy question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium sulphate?) 1)Casein Isolation from MilkExperimental Procedure* Put 100 ml of milk in a 500 ml beaker and heat it to 40 0C. * Also heat 100 ml acetate buffer and slowly add it to the beaker with milk while stirring. * The final pH of the mixture should be 4.8. * Cool the suspension to room temperature and wait 5 more minutes before filtering. * Wash the precipitate several times with small volumes of water and then suspend it in approximately 30 ml of ethanol.Filter the suspension in the buchner funnel and wash the precipitate a second time with a mixture of equal proportions of ethanol and ether. * Finally, wash the precipitate on filter paper with 50 ml of ether and soak and dry. * Make the ether evaporate by spreading the powder. * Weigh the casein and calculate the percentage yield of the protein. * Compare with theoretical yield of 3.5 g per 100 ml milk. 2)Purification of Egg…
- Inoculate 250-µL overnight cell culture into 50 ml LB medium (in a 250 ml flask). Shake vigorously at 37 °C to OD600~0.5-0.6 (usually it takes about 2-3 hours). Why should we use a larger flask during culture at this step? Why do we need to wait till this OD range is achieved?Two flasks of E. coli are grown in batch culture in the same medium (2% glucose and amino acids; no nitrate) and at the same temperature (378C). Culture #1 is well aerated. Culture #2 is anoxic. After 16 hours the following observations are made: ■ Culture #1 has a high cell density; the cells appear to be in stationary phase, and the glucose level in the medium is reduced to 1.2%. ■ Culture #2 has a low cell density; the cells appear to be in logarithmic phase, although their doubling time is prolonged (over 1 hour). The glucose level is reduced to 0.2%. Why does culture #2 have so little glucose remaining relative to culture #1, even though culture #2 displayed slower growth and has less biomass?The arrowhead shaped zone shows what reaction? How do you set up the test for this reaction? For which organism is this an important test?