Compute for the appropriate amount (mL) of the compostions below of CTAB precitpitation solution used for DNA extraction. a. 1% w/v CTAB b. 10 mM EDTA c. 50 mM Tris-Cl
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Compute for the appropriate amount (mL) of the compostions below of CTAB precitpitation solution used for DNA extraction.
a. 1% w/v CTAB
b. 10 mM EDTA
c. 50 mM Tris-Cl
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Solved in 3 steps
- Following the monograph procedure, a 724-mg of aspirin (MW=180 g/mol) dissolved in 18.5 ml of cold neutralized alcohol. This solution was then initially titrated with 0.101 N sodium hydroxide solution, then later neutralized with 0.104 sulfuric acid. 1. What is the total volume (mL) of excess titrant used in the titration? a. 56.400 mL b. 52.000 mL c. 44.600 mL 2. What is the milliequivalent weight consumed by the acidic titrant? a. 1.5392 g-meq b. 2.0907 g-meq c. 1.4948 g-meq d. 2.1528 g-meq 3. What is the milliequivalent weight consumed by the basic titrant? a. 5.8656 g-meq b. 1.5392 g-meq c. 5.6964 g-meq d. 1.4948 g-meq 4. What is the difference of milliequivalent weight consumed in the reaction? a. -4.1572 g-meq b. 4.3708 g-meq c. 0.5515 g-meq d. 4.1572 g-meq 5. What is the percentage purity of the sample? 6. What is the titer value (mg) based from the specific type of titration?3. You are loading a protein gel that holds up to 50ul. You need to load 25ug of protein sample that is at a concentration of 8 ug/ul. You are mixing your sample with a 4x Laemmli Buffer prior to loading. How much of each component will you mix and how much of total volume will you load in the well? You-a- EL COL DDOT.no:9. wo samples of the same soil is subjected to pH analysis using a pH meter, the first is analysed using 1:1 soil:water ratio and the other with 1:1 soil:KCl ratio, what would be the most probable result? A. Soil analused using 1:1 soil:KCl ratio will register a higher pH compared to that of soil analysed using 1:1 soil water ratio B. Soil analysed using 1:1 soil:KCl will register a lower pH compared to that of soil analysed using 1:1 soil:water ratio C. Soil analysed using 1:1 soil:water ratio will register a lower pH compared to that of soil analysed using 1:1 soil:KCl ratio D. Soil analysed using 1:1 soil:water ratio will have the same pH with that of soil analysed using 1:1 soil:KCl ratio
- Which is the correct Ksp expression for PbCl2 (s) dissolving in water? Select one: O A. Ksp = [PbCl*] [CI %3D O B. Ksp - [Pb2+j² [CI] %3D [Pb2+] [CI] [Pb2+] [C}? C. Ksp %3D O D. Ksp %3D O E. Kgp = [Pb*] [C?72 [Pb*] [Ci?72 %3DYou run a DEAE-cellulose column to purify anions from a biological sample. Which approach, from the options listed below, would you take to elute the bound fraction? Apply a buffer of decreased anionic strength to the column. а. Apply a buffer of increased anionic strength to the column. b. Apply the equilibration buffer to С. the column. Apply a buffer of increased pH to the column. d.Following the monograph procedure, determine the weight in grams of sodium carbonate (MW-106 g/mol) used to standardize a 0.987 N sulfuric acid solution. 1. Consider that the burette was completely filled to the 0 mL mark before titrating. What is the volume of titrant consumed based from the image below? A. 22.9 mL B. 21.2 mL C. 21.3 mL D. 22.7 mL 2. What is the unknown weight (grams) in the problem? Your Answer:
- Is prepared by dissolving a definite weight of a substance in a definite volume. a. primary standard b. secondary standard c. standard addition d. standard sample e. standard solutionA protein of molecular weight = 55,000 g/mol and at 1 mg/mL concentration was titrated with Ellman’s reagent. What is the molar concentration of the protein in solution?09. Two samples of the same soil is subjected to pH analysis using a pH meter, the first is analysed using 1:1 soil:water ratio and the other with 1:1 soil:KCl ratio, what would be the most probable result? A. Soil analused using 1:1 soil:KCl ratio will register a higher pH compared to that of soil analysed using 1:1 soil water ratio B. Soil analysed using 1:1 soil:KCl will register a lower pH compared to that of soil analysed using 1:1 soil:water ratio C. Soil analysed using 1:1 soil:water ratio will register a lower pH compared to that of soil analysed using 1:1 soil:KCl ratio D. Soil analysed using 1:1 soil:water ratio will have the same pH with that of soil analysed using 1:1 soil:KCl ratio
- Submit a clean, dry, and properly labeled 50-mL reagent bottle for your unknown solution. Pipet 20.00 mL of the sample in 250-mL Erlenmeyer flask. Add 5 mL of buffer and 5 drops of indicator. Titrate the solution until it turns light blue. If the titration consumes more than 50 mL of the titrant, dilute the sample accordingly. Compute for ppm of CaCO3 using the following table. Mean Molarity = 2.487 x 10^-3 MBlank correction = 0.015 Formula = V (mL) titrant x Mean Molarity of Titrant x Molecular weight of CaCO3 x 1/V (L) sample1. What is the pH of a buffer that contains 1.100 M NH3 and 1.300 M NH4+? Ka= 5.70x10-10What would be the new pH if a 100 mL portion of 0.075 M HCl is added to another 400 mL portion of the buffer? 2. If a multivitamin was manufactured on March 2021, when is its most likely expiration date?a. June 2021b.December 2021c. September 2021d. March 2022A student performed titrations of samples of borax at different temperatures to determine the KSP at each temperature. After graphing the 1/T (K-1 ) versus ln(KSP), the student obtained the trend line and R2 value. Use this information to answer the following questions: a. What is deltaH? b. Is this dissolution endothermic or exothermic? c. What is deltaS? d. Determine deltaG at 298K.