Consider the following microscope: ocular power = 10X low power objective = 20X high power objective = 50X 4. %3D What is the highest magnification you could get using this microscope? If 10 cells can fit end to end in the low power field of view, how many of those cells would you see under high power?
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- Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
- 4. If the diameter of the field of view (FOV) for your microscope is 5.5 mm at 40x total magnification, what is the diameter of the FOV at 1000X? Show work and don't forget units! 5. Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don't forget units! Elodea Leaf at 400x total mag 6. Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use your calculation for the FOV at 1000x total magnification. Show work and don't forget units! Cell Membrane Nucle usUsing the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?If you are using a microscope and a specimen takes up 1/4 of the field of vview at 1000X, what is the diameter?
- a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?What is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?
- L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperture1. What does moving the coarse focus and fine focus do? Why is it important to remember the difference between coarse and fine focus? 2. What is the resolution of a microscope? How is resolution affected byadjusting the light, coarse and fine focus? please answer on ur own words, do not copy from google or i will downvote