Construct an explanation for each step of DNA Isolation.
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- 2. Who are the scientists that proved DNA is the transferrable genetic material and Discuss how they proved based on their experiment?4. Explain why Maurice Wilkins and Rosalin Franklin needed to use X-rays (instead of visible light) when "taking pictures" of crystalized DNA.4. Explain the importance of Isolating DNA from the cells of organisms.
- 4. In bacterial cells, nucleotide excision repair involves which of the following proteins?A. DNA glycosylaseB. AP endonucleaseC. photolyaseD. AlkBE. UvrABC proteins 5. If Meselson and Stahl had results from density gradient analysis of bacterial DNA that indicated only two bands, one of the original density and one that was the same as unlabeled DNA, and no intermediate density band, this would indicate that DNA replication is:A. constructiveB. semiconservativeC. conservativeD. consecutiveE. cannot determine from the information given 6.In E. coli, which DNA polymerase is primarily responsible for filling in the gaps in the DNA generated during nucleotide excision repair?A. DNA polymerase IB. DNA polymerase IIC. DNA polymerase IVD. DNA polymerase VE. none of the above13. In your own words, identify one of the reasons why it is difficult to assemble a genome and explain why .1. How can you modify your DNA model to reflect changes among DNA molecules? 2. How can a DNA molecule make an exact copy of itself?
- What information and materials are needed to amplify region of DNA using PCR?6. Describe the experiments that were done by Meselson Stahl to distinguish between conservative, semi-conservative and dispersive DNA replication. Include the various hypotheses they were testing and their conclusions.2. How does the universality of the genetic code make the recombinant DNA technology possible?
- 2. Describe what is meant be the antiparallel arrangement of DNA.4. Meselson and Stahl's experiments examined DNA after 0, 20 and 40 minutes. Complete the table below to show the percentage of the DNA density after 60 minutes, assuming a) conservative replication and b) semi-conservative replication. Conservative replication Semi-conservative replication Time (mins) 14N/ 14N (light) 14N/15N (intermediate) 15N/15N (heavy) 40 60 40 60 75% 50% 50% 25% 5. What temperatures (°C) are typically used for each step of PCR? Denaturation Anneal primers DNA synthesisA. Illustrate. Consider the given pair of homologous DNA molecules. T T' t t' Y Y' y y' L Ľ' I l' G G' g g Suppose the single strand breaks in the figure initiated an exchange of segments, and branch migration proceeded up to the L'-G' region. Following the color-coding and genotypes of the given DNA molecules,