Define nucleic acid. What are the best sample sources that can be used in the extraction of nucleic acids? Give examples (2) of nucleic acids. Differentiate RNA from DNA in terms of their chemical properties.
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Q: Give information about the methods based on nucleic acid hybridization? in details
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A: DNA extraction is a process involve disruption and lysis of the cell followed by the removal of…
Q: What is the purpose of precipitation?
A: 1. DNA is the genetic material present in most of the living organisms. The DNA is made up of 4…
Q: Describe the components vital for DNA sequencing with the Sanger method
A: DNA sequencing: The process of identifying the correct order of the four nitrogen bases (adenine,…
Q: Name the technique used to separate fragments of DNA or mixtures of proteins?
A: The gel electrophoresis is a technique to separate DNA fragments and proteins based on their size,…
Q: Once DNA is extracted, what can it be used for?
A: Genetic material is the molecules which should have certain characteristics like it should replicate…
Q: Provide detailed explanation of the following super-resolution techniques: GSD, RESOLFT
A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…
Q: Would you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis? Why…
A: Gel electrophoresis is the technique where macro molecules especially proteins and nucleic acid are…
Q: In the extraction of DNA of Banana, what do the DNA look like?
A: Introduction Bananas are elongated, edible berries produced by several species of big herbaceous…
Q: Which of the following is true regarding next generation sequencing? 1. Next generation sequencing…
A: Answer Next generation sequencing is a powerful platform that has enabled the sequencing of…
Q: When DNA probes are used to detect specific sequence similarities in bacterial DNA, the probe is…
A: The DNA probes can be single or double-stranded containing sequence-specific oligonucleotides.
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: When you isolate DNA, what is the purpose of the hot water and the detergent in the activity?
A: The basic principle related to DNA isolation is to distrup the cell wall, cell membrane and nuclear…
Q: Nucleic acids involved in protein synthesis include DNA sense strand DNA anti-sense strand mRNA…
A: Nucleic acids are composed of phosphoric acids, sugars, and mixtures of organic bases like purines…
Q: What is a DNA extract?
A: DNA or deoxyribonucleic acid is a biomolecule that serves to act as genetic material for most of the…
Q: if the helix is more stable in water, would there be a way to synthetically create base pairs in…
A: Double stranded DNA is composed of two complementary strands with each strand consisting of sugar…
Q: What factors would contribute to preservation of DNA?
A: Introduction DNA preservation (also known as DNA banking):- It allows you to store some of your…
Q: How does a dideoxynucleotide differ from a deoxynucleotide? How are dideoxynucleotides important in…
A: Dideoxynucleotides are DNA polymerase chain-elongating inhibitors used in the Sanger technique of…
Q: Can DNA be isolated from beef? Discuss the process briefly
A: The purification of DNA from the collected sample of DNA with the help of the various methods and…
Q: hy wasn’t James Watson punished by the scientific community once more was learned about how he…
A: Watson and Crick started their work on 1950 -1952 . They proposed three stranded model and…
Q: Match the following functions to each of the enzymes listed below. Some Functions of Enzymes Build…
A: DNA is the nucleic acids present in the organisms. DNA is the deoxy-ribose nucleic acid in which…
Q: Unusual nucleotides, like ribothymidine and dihydrouridine, can normally be found in which of the…
A: Nucleotides are the monomeric units of the genetic material that forms the nucleic acid structure.…
Q: How griffin's experiment helps in the identification of DNA as the genetic material?
A: Introduction In the past years when the genetic and molecular biology techniques were not so…
Q: What is the experiment that helped Hershey and Chase recognize DNA as a genetic material? Explain in…
A: Introduction: The undeniable proof that DNA is the genetic material reached from the experiments of…
Q: Describe the role of deoxyribonucleotides in generating DNA fragments for analysis?
A: DNA is hereditary material in most of the living organisms. DNA is long polymer of…
Q: In DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic…
A: Silica based DNA extraction method is an easy and economical method for extracting nucleic acid. It…
Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…
A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…
Q: How is the absorption of ultraviolet light by DNA and RNA importantin the analysis of nucleic acids?
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
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A: Cells contain nucleic acid which stores essential genetic information for the survival of the cell.…
Q: What is used to identify the size of a DNA molecule during gel electrophoresis?
A:
Q: What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the…
A: The 260 nm light detect the sugar- phosphate backbone of DNA when measuring the purity of a DNA…
Q: The most common elements in living organisms are carbon, hydrogen, oxygen, nitrogen, phosphorus, and…
A: The macromolecules essential to life are formed using these common elements such as hydrogen,…
Q: How can you differentiate the types of nucleic acids? Make a comparison table. DNA RNA Number…
A: Nucleic acids are long chain polymeric molecules . The monomers is known as nucleotides. Hence the…
Q: What are the main types of nucleic acids? Differentiate nucleic acids, nucleotides, and…
A:
Q: In the isolation of DNA which of the following best describe the purpose of using NaCl-SDS solution?
A: DNA extraction is a technique for separating DNA from cell membranes, proteins, and other cellular…
Q: What is the results of extracting the DNA of a strawberry?
A: Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and…
Q: What types of DNA can be extracted? Explain your answer.
A: Some of the most common DNA extraction methods include organic extraction, Chelelx Extraction and…
Q: describe techniques used to identify and quantify specific dna sequence in complex mixtures
A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil…
Q: What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?
A: Fluorescent labeling and radioactive labeling have found wide applications in DNA sequence analysis…
Q: . What information about the structure of DNA wasobtained from X-ray crystallographic data?
A: The deoxyribonucleic acid (DNA) is a hereditary material that is found in almost all living…
Q: .What methods are used to visualize radioactively labeled nucleic acids?
A: Electrophoresis is a laboratory method that uses an electric field to separate molecules. The charge…
Q: Write down eight facts about nucleic Acids DNA and RNA?
A: Introduction Nucleic acids are biopolymers, or large biomolecules,essential to all known forms of…
Q: What are the different methods of quantifying DNA? How would you determine if your
A: Quantitation of DNA ,methods used for quantifying DNA ---* Quantifying of DNA -- In various methods…
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- When separating proteins from cellular extractions, what electrophoresis method works best and what protein characteristics need to be taken into account that are not a problem for nucleic acid-based molecule? Describe the process and in your answer.Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.
- You have an aqueous solution containing: Alaine (a mono-amino, monocarboxylic acid) Fructose (a non-ionic monosaccharide) Glycogen (a non-ionic large polysaccharide) Ribose-5-phosphate (an anionic monosaccharide phosphate) t-RNA (a polyanionic nucleic acid, MW~ 30,000). Assuming you have a distinctive assay for each of these compounds, what procedures would you use to obtain gram quantities of each of these compounds free of each of the other compoundsSketch the appearance after visualization of a protein mixture containing the seven proteins above when subjected to two-dimensional (2D) gel electrophoresis.RNA can be hydrolyzed by dilute alkali but not DNA. Why?
- briefly explain the physical mechanism by which amino acids are separated during paper chromatography.give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. higher absorbance at 260nm of melted DNA than the native DNAElectrophoresis is an extremely useful procedure when applied to analysis of nucleic acids as it can resolve molecules of different sizes with relative ease and accuracy. Large molecules migrate more slowly than small molecules in agarose gels. However, the fact that nucleic acids of the same length may exist in a variety of conformations can often complicate the interpretation of electrophoretic separations. For instance, when a single species of a bacterial plasmid is isolated from cells, the individual plasmids may exist in three forms (depending on the genotype of their host and conditions of isolation): superhelical/supercoiled (form I), nicked/ open circle (form II), and linear (form III). Form I is compact and very tightly coiled, with both DNA strands continuous. Form II exists as a loose circle because one of the two DNA strands has been broken, thus releasing the supercoil. All three have the same mass, but each will migrate at a different rate through a gel. Based on your…
- What is the structure of the gel stain being used? There are several other dyes that can bind DNA and used for gel imaging. Find an example of such a dye, and draw its structure.A mixture of amino acids that do not separate sufficiently when a single technique is used can often be separated by two-dimensional chromatography. In this technique, the mixture of amino acids is applied to a piece of filter paper and separated by chromatographic techniques. The paper is then rotated 90°, and the amino acids are further separated by electrophoresis, producing a type of chromatogram called a fingerprint. Identify the spots in the fingerprint obtained from a mixture of Ser, Glu, Leu, His, Met, and Thr.How can intact DNA be obtained from a solution of DNA and RNA?