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Q: Chromatography
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Q: The plot of retention time versus detector signal in chromatography called?
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A: All the Above.
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Q: by
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Q: For what type of separation would you use ion exchange chromatography (IC)?
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Define the following terms used in HPLC:
(d) sampling loops
(e) bonded-phase packing
(f) gel filtration
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- Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography (d) Use three diagrams to illustrate the separation of three different proteins by thesemethods.In have narrow peaks are said to chromatography, a) a large plate height (H) b) a small plate height (H) c) a small number of theoretical plates (N)Help me.... Explain the principles of gel permeation chromatography in Polymer, what is the application in Polymer analytics
- Can you describe the main pathways (ingredient class or method) for modifying the rheological properties and adding viscosity / yield to a surfactant cleansing system (shampoo)? What would you be doing to the micelle and why would it thicken?4.Discussed the Purposed of the Golden rules in the practice of Forensic Chrmistry.Principles of separation by capillary Isolachophoresis
- Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?(a) The figure that follows is an idealized enlargement of the region near the cinnamaldehyde peak. Determine the retention time for cinnamaldehyde. (b) From the figure in part (a), determine the number of theoretical plates for the column.Why acetone has les retention time and toluene has more. discus about polarity of 3 samples and interaction with stationary phase
- The following data were obtained for four compounds separated on a 20 m capillary coloumn. Compound t,(min) w(min) A 8.04 0.15 B 8.26 0.17 C 8.43 0.18 (a)Calculate the number of theoretical plates for each compound and the average number of theoretical plates for the column. (b) calculate the average height of theoretical plates. (c) explain why it is possible for each compound to have a different number of theoretical plates?describe different type of degasser in HPLC system, and why degassing is important in the middle of HPLC operation?In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.