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Q: ACAACCCCAAGCCTTC
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Describe how TaqMan probes can specifically measure human DNA concentration, but SYBR Green I cannot-explain.
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Solved in 2 steps
- Ehat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatgwhy does a higher agarose concentration render better resolution/separation of smaller DNA fragments? and what determines the distance of DNA fragments in gel electrophoresis?Using a ThermoFisher GeneJet Miniprep plasmid isolation kit to isolate plasmids from bacteria: Use of RNAse increases purity of purified plasmids. This enzyme will be added when the cells are lysed, detroying all RNA in the sample. Why will this action increase the purity of an isolated plasmid sample? This will only be used for the plasmids isolated using the GeneJet purification columns, and not when performing alkaline lysis. How will this affect the predicted results for the alkaline lysis plasmid isolations?
- Describe the rationale for column-based DNA extraction and purification.Compare and contrast the principle behind DNA migration in agarose gel electrophoresis from that of protein migration in SDS-PAGE.Do virtual restriction digests of 300ng pGLO plasmid using HinDIII, EcoRI, and XhoI separately, and a double digest of pGLO with HinDIII plus EcoRI. How many nanograms of DNA should we expect to be contained within the slowest bandfrom the HinDIII-digest of pGLO?
- What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveWhat are DNA probes Describe three (3) methods that can be used to label DNA probes Describe 2 methods for detection of the hybridized probe List the advantages of using non-radioactively labeled probes vs radioactively labeled probes.How is plasmid DNA distinguished from coli chromosomal DNA using the Qiagen Miniprep protocol? Please provide citation of used