Question 3. Dihydrofolate reductase (DHFR) is capable of making one carbon moieties of variousoxidation states (i.e., methanol, formaldehyde, formate). Folic acid, a B vitamin, provides the criticalchemistry:This type of chemistry is utilized in the synthesis of nucleotides. Methotrexate has a significantlyhigher affinity (Kd ~ 50 pM) than folate for the enzyme and is used in cancer therapy to suppressDNA synthesis of rapidly dividing cancer cells. What is a tempting reason for methotrexate havingsuch high affinity?(b)CH:H2N.CH2CH,N-C-HNH2C2-Amino, 4-amino analogs of folic acidR = HR - CH, Amethopterin (methotrexate)AminopterinDoes your suggestion change when told that crystallography determined that methotrexate binds tothe protein with opposite orientation to folate? (two sentences should do it)HNFolateNADPH + HNADP"N°HDihydrofolateNADPH+H INI7 +daVN

For cancer Methotrexate competitively inhibits dihydrofolate reductase, an enzyme participate in the…

Answered: Dihydrofolate reductase (DHFR) is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Phosphorylated molecules with high _____________ values of hydrolysis have large phosphoryl group transfer potentials.
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Question 3. Dihydrofolate reductase (DHFR) is capable of making one carbon moieties of various oxidation states (i.e., methanol, formaldehyde, formate). Folic acid, a B vitamin, provides the critical chemistry: R. This type of chemistry is utilized in the synthesis of nucleotides. Methotrexate has a significantly higher affinity (Kd - 50 pM) than folate for the enzyme and is used in cancer therapy to suppress DNA synthesis of rapidly dividing cancer cells. What is a tempting reason for methotrexate having such high affinity?
Question 4. Diabetes mellitus possesses a serious health challenge worldwide and there has been an increased awareness for self-monitoring and point of care testing. The development of self- monitoring of blood glucose (SMBG) is probably the most important advance in controlling diabetes since the discovery of insulin. a) Dextrostix, the first glucose meter was invented by Ernie Adams. Briefly discuss. b) There are three principles of enzymatic reactions, utilized by current glucose meters. Compare and briefly discuss these three principles.
question 1 : draw an α,α(2, 2) linkage between the two monosaccharides(two fructose ). label the glycosidic linkage. Must be Haworth question 2 : Draw glycosidic linkage between D-glucose linked to D-altrose with a β(1, 3) glycosidic linkage. label clearly the glycosidic linkage. must be Haworth.
When a purine or a pyrimidine is linked through a β-N-glycosidic link to C-1 of a pentose, the molecule is called a _____________ .
Question 11 Observe the titration curve below and choose the CORRECT statement. 12 - pK2 10 8- PK, 2. 0.5 1.0 1.5 Equivalents of OHO 2.0 O Two of these are correct O At point A, both acidic groups are protonated O At point A, both acidic groups are deprotonated O Cis the end point of titration for the most acidic group O B is the end point of titration for the most acidic group « Previous N. LG rotein d (basic)Rgoup Jacidic) Rgroup ged Rgroup
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Question 1. Put away your notes and text and draw the complete Calvin-Benson cycle starting from 3molecules of Ru1,5BP. Show stoichiometry along the way using 6 GAP molecules to regeneratethe 3 Ru1,5BPs. Label each reaction according to its enzyme class (your choices arecarboxylase, kinase, dehydrogenase, aldolase, epimerase, isomerase, phosphatase, andtransketolase – see the posted enzyme guide for help on this).
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Question 5 of 40 Side-by-Side Identify the charge states for aspartic acid (Asp), leucine (Leu) and Lysine (Lys) at pH 1, 7 and 12. Then calculate the isoelectric point (pI). Step 7: Determine the appropriate calculation for the pl of lysine. For lysine, the pKa of the amine is 8.95, while the pKa of the carboxylic acid is 2.18 and the pK, of the amine in the Strategy: First identify the sidechain on each amino acid. Then determine whether the side chain is acidic, basic or side chain is 10.53. neutral. Determine whether each functional group is Calculate the pI of lysine. charged or not at the pH indicated. Calculate the (pI) from the pKa values of the functional groups. pI MacBook Pro

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Dihydrofolate reductase (DHFR) is capable of making one carbon moieties of various ates (i.e., methanol, formaldehyde, fgmate). Folic acid, a B vitamin, provides the critical