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Does the addition of E. coli change the dilution in the calculation of PFU/mL in the original phage sample? Why/Why not?
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- Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?What are the defining characteristics of the exponential/log phase of the bacterial growth curve. Explain the culture conditions that arise that cause the phase to end. In different culture media, and the exact same starting inoculum, will the end of exponential/log occur at the same time? Why or why not? A graph or a drawing of the growth curve would help but is not essential to answer this question.
- TRUE OF FALSE: Is E coli colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)? Is Coliform colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)?A culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.How long does it take for E. coli to go from lag phase at time 0 to log phase to stationary phase to death phase? Can you point me to a citable source of this time period? I can't find anything online. Thank you.
- When T4 phage isolated from E. coli strain B infect E. coli strain K, what will be the EOP (efficient of plating)? Explain why. Fe VUse the following data to calculate the original bacterial sample. 1 ml Sample of E. coli 1/10 OF-10 9 ml 1 ml 240,000,000 15,200,000 DF = Dilution Factor 152,000,000 24,000,000 1-7000 OF-100 9 ml 1/1003 OF-1000 9 ml 1/7101.000 1/333/00 1/1,800,330 OF-11003 OF-100.000 OF-1,000.JINI 9 ml Over 400 152 9 ml 1 ml 24 1/11,000 1/933.000 1/1/000.000 OF -10,330 OF-TEXLIED DG-1,000,000Pseudomonas bacteria have porin proteins, are resistant to the chemical triclosan, and survive and can even multiply in quaternary ammonium compounds. True or False?
- you are given a mixed culture of S. aureus, E. coli and P. aeruglinosa. How would you isolate each of them from this mixed culture? Besides usina a streak plateTRUE OF FALSE: Is E coli colonies colorless in Eosin Methylene BlueAgar (EMBA)? Coliform colonies colorless in Eosin Methylene BlueAgar (EMBA)?mited Culture of StrainI + Grouh is representel by a Squiggly cone of inoculation Strain TI Str I mix Strit StRI mix Ampiaillin gentamycin Str mix ampicillin gentamycin