Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter28: Atomic Spectroscopy

Section: Chapter Questions

Problem 28.4QAP

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Question

Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia
tinctorum, commonly known as rose madder. Earth spotted the root extract on two
hydrophobic silica gel plates. He then placed each plate in separate developing chambers,
one containing solvent system WHITE and the other housing solvent system MIX. Earth
allowed the chromatograms to develop for around five minutes. Afterwards, he removed
the plates from the containers and placed them inside an iodine chamber. The
chromatograms resulting from the TLC experiment after visualization are illustrated as
follows. The distances travelled by each spot and by the solvents were measured and
recorded in the displayed table.

D
75.0 mm
71.5 mm
DISTANCE
SOLVENT
SPOTS
TRAVELLED FROM
D
SYSTEM
THE ORIGIN, mm
13.0
32.5
solvent
front
A
В
WHITE
41.0
D.
A
B.
66.5
10.5
В
27.5
MIX
origin.
39.0
A
D.
53.5
10.5 mm
8 mm
WHITE
MIX
0 mm.
O mm

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