Plate 2: Staphylococcus aureus Diameter 35 mm A Diameter 25 mm D Disinfectant Pseudomonas aeruginosa B Diameter 30 mm C Diameter 27 mm Zone of Inhibition (mm) A B C D (Chlorine) (Lysol) (lodine) (Alcohol) Staphylococcus aureus 1.Record the zones of inhibition for each bacterial species tested in the chart above. 2. Which disinfectant is most effective against a) the Gram positive bacterial species tested? b) the Gram negative bacterial species tested? Ex. 16: Disinfectants and Antiseptics The bacteria tested were Pseudomonas aeruginosa (Gram negative) and Staphylococcus aureus (Gram positive). Sterile filter paper disks soaked in disinfectants were placed on the bacterial lawn as described on page 61 in the lab manual. Both plates were incubated at 37°C for 18-24 hours. Key A-Chlorine B-Lysol C-Iodine D-Alcohol Disk with disinfectant (see labels) Area with no bacterial growth Bacterial growth Plate 1: Pseudomonas aeruginosa Diameter 20 mm A. B Diameter 15 mm Diameter 13 mm C Diameter 10 mm
Plate 2: Staphylococcus aureus Diameter 35 mm A Diameter 25 mm D Disinfectant Pseudomonas aeruginosa B Diameter 30 mm C Diameter 27 mm Zone of Inhibition (mm) A B C D (Chlorine) (Lysol) (lodine) (Alcohol) Staphylococcus aureus 1.Record the zones of inhibition for each bacterial species tested in the chart above. 2. Which disinfectant is most effective against a) the Gram positive bacterial species tested? b) the Gram negative bacterial species tested? Ex. 16: Disinfectants and Antiseptics The bacteria tested were Pseudomonas aeruginosa (Gram negative) and Staphylococcus aureus (Gram positive). Sterile filter paper disks soaked in disinfectants were placed on the bacterial lawn as described on page 61 in the lab manual. Both plates were incubated at 37°C for 18-24 hours. Key A-Chlorine B-Lysol C-Iodine D-Alcohol Disk with disinfectant (see labels) Area with no bacterial growth Bacterial growth Plate 1: Pseudomonas aeruginosa Diameter 20 mm A. B Diameter 15 mm Diameter 13 mm C Diameter 10 mm
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 2.4P
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3. Which disinfectant would you choose if you were trying to kill both bacterial species? (In other words, which chemicals works the most effectively against both species tested?)
4. Which disinfectant is the least successful against each bacterial species? (You should have an answer for the least effective against Gram positives and an answer for the least effective against gram-negatives- it may or may not be the same chemical.)
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