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- Explain the term gradient elution mode in HPLC Differentiate between gel filtration and gel permeation chromatography.Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a necessary technique in HPLC? What are the steps involved in gradient elution?Which of the following solvents is used in normal phase elution in HPLC? a) Ethanol: Water b) Hexane c) Acetonitrile CH;CN d) Methanol
- What are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in HPLC?Enumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.describe different type of degasser in HPLC system, and why degassing is important in the middle of HPLC operation?