Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfate

Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfate

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 9QP: Define and compare the following types of nucleotide substitutions. Which is likely to cause the...

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Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfate
Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action 2-aminopurine Acridine orange Proflavine
Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Heat 5-bromouracil Ethidium bromide
The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1) RNaseH
Give the SIGNIFICANT difference between the terms provided in the table below. Somatic mutation Germline mutation Turner’s Syndrome Down Syndrome Muscular Dystrophy Burkitt’s Lymphoma Deletion Duplication Translocation Inversion Transition Transversion Missense nonsense Point Mutation Frameshift Mutation Intercalating Agents Alkylating Agents Based Excision Repair Mismatch Repair
Explain how site directed mutagenesis can be accomplished using M13 bacteriophage. Using this method often results in only 5% of plaques with mutant gene. Explain how the procedure has been modified to enrich for the number of plaques with the mutant gene.
With reference to the image below, discuss the process and principle involved for screening/selection of hosts (last stage of cloning) containing the intended recombinant plasmid. LacZ' = Gene for alpha-peptide of β-galactosidase.
Explain in detail the AMES test which is used to test for the mutagenic potential of different compounds. Include an explanation of the AMES tester strains and how they work, why the liver is essential to this test and specifically why the control plate is necessary.
Give the SIGNIFICANT difference between the terms provided in the table below. NO NEED for complete sentences, phrases will do. Somatic mutation Germline mutation Turner’s Syndrome Down Syndrome Muscular Dystrophy Burkitt’s Lymphoma Deletion Duplication Translocation Inversion Transition Transversion Missense nonsense Point Mutation Frameshift Mutation Intercalating Agents Alkylating Agents Based Excision Repair Mismatch Repair
Enumerate the steps involved in gene cloning and the protein/enzyme requirements for each step. Just put the short but concise answer and kindly avoid plagiarism. Thank you.
A scientist wants to make a random mutation in a specific gene to investigate that gene's function. Which of the following methods is she likely to have used to make this change? mutagenesis with radiation random mutagenesis CRISPR-Cas9 genome editing followed by nonhomologous end joining (NHEJ) CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template oligonucleotide-directed mutagenesis
Cloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.