The following data were obtained from the analysis of a mixture of compounds (A-D) byHPLC:PeakRetention time (min) Width of peak base (min)non-retained 2.1A.5.410.312.10.511.031.25C20.61.62a) Using peak C, calculate the number of plates (N) in this column and the plate height (H)for the column.b) For peak D, calculate the capacity factor (k').c) For peak B and C, calculate the resolution (R.).

The full form of HPLC is high performance liquid chromatography. It is used to identify or separate…

föllowing analysis of a compounds (A-D) by were obtaine HPLC: Peak Retention time (min) Width of peak base (min) non-retained 2.1 A 5.4 0.51 10.3 1.03 C 12.1 1.25 D 20.6 1.62 a) Using peak C, calculate the number of plates (N) in this column and the plate height (H) for the column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C. calculate the resolution (R.).

föllowing analysis of a compounds (A-D) by were obtaine HPLC: Peak Retention time (min) Width of peak base (min) non-retained 2.1 A 5.4 0.51 10.3 1.03 C 12.1 1.25 D 20.6 1.62 a) Using peak C, calculate the number of plates (N) in this column and the plate height (H) for the column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C. calculate the resolution (R.).

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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