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- Question 4 a) Explain the importance of reducing exposure dose and time to a patient undergoing a diagnostic X-ray examination. Describe ways to achieve this. b) Calculate the wavelength of the most energetic X-rays produced by tube operating at pd of [1.4]Question 5 a) Explain the general diagnostic principles using radiopharmaceuticals by which medical images may obtained. b) Describe the use of technetium-99m-MDP. c) Describe the use of 1251 radioisotope. [Suggested word count 270] [2.2]Question 1 Which application are micropipettors NOT commonly used for? O 1. Mixing/resuspending cells or reagents into solution. 2. Transferring microliter (µL) volumes of liquid between receptacles. O 3. Measuring milliliter (mL) volumes of liquid. O 4. Loading samples for analytical techniques.
- QUESTION 1 A metric ruler was placed under the microscope and using the low-power objective (10x), a student measured a specimen to be 1.2 mm. a) Convert the diameter at low power (10x) to micrometers (µm). b) Calculate the high-power (40x) diameter field of view using the information from part a). Answers must contaln proper units for full credit.Question 2 The Kirby-Bauer disk diffusion method is often used in clinical diagnostic labs. Question 2 options: a) True b) FalseQuestion 3 Describe the key properties required of radioisotopes used for i) medical imaging (gamma camera, PET scans) ii) brachytherapy (e.g. radioactive implants) iii) internal 'systemic' radiotherapy (e.g. lodine-131 thyroid cancer treatment) [Suggested word count 200] [1.3]
- Question 3 a) Explain the key properties Tc 99m used for medical imaging. b) Explain how you store such radioisotope. [Suggested word count 200] [1.3]Question 5: The following experiment was done to separate plant pigments from spinach leaves. 4 3 Watch glass containing concentrated extract a. Fill in the information in the table below: Pigment 2 No. 1 Lost the sample Name Solvent Front Det up the chromatography chamber Allow the solvent to move up Color Olive Green Blue Green Yellow Yellow-Orange Sample apot Distance travelled (cm) 3 5.6 8.4 13.5 15 What is the name of this technique for separation of the pigments? Solvent From Pigment 4 0.2 Pigment 3 Pigment 2 Ratio factor (Rf) b. Write the equation and show the calculations to find out the Rf for pigment 3. Pigmen 1 0.37 0.9 n that the solvent used was non-polar, which of the pigments is the most polar?Question 5 For a successful direct staining, one should A. use a minimal amount of water to speed up the air-drying step B. make a very thick smear of cells on a slide to facilitate finding the cells under a microscope C. heat-fix a smear for an extended time to ensure that the cells will be stuck firmly to the slide D. All of the above E. A and C
- QUESTION 1 What is 105 ng ml in: a. ug/ml b. mg 1 C. ug1 d. g/ul (Please list each answer on a separate line, beginning with the corresponding letter. Don't forget units for each answer!) For the toolbar, press ALT+F10 (PC) or ALT-FN+F10 (Mac). 14px E E YA BIUS Paragraph Arial 田 Bヨ田田用 国 田EEt oQuestion 1 Inoculation for bacterial growth in an agar petri dish requires A. Incolating one species of bacteria per dish B. Host tissue so the bacteria can grow inside C. That the agar have nutrients D. Incubation in proper temperaturesQUESTION 9 In a broth dilution test, the first clear tube in the dilution series indicates: O a. thermal death time b. best antibiotic for the situation OG MIC d. zone of inhibition QUESTION 10 Who is credited for first finding a naturally occurring antimicrobial agent? O a. Joseph Lister Ob. Edward Jenner OC. Alexander Fleming Od. Paul Ehrlich