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- Regarding TLC plates: a) When spotting a TLC plate, are small spots or large spots better? Or does the size of the spots not matter? b) Do separation of compounds on a TLC plate depends primarily on polarity, boiling point, molecular weight, melting point, or density?The TLC and mp data contradict one another with regards to purity. True FalseDescribe the fundamental principle of all PtX technologies. What is the unifying technology requirement?
- Please choose an appropriate technique(s) to separate following compounds (it can be one, more than one, on none): Compound Melting Point Density Boiling Point Molar mass Phenol 40.5°C 1.07 g/cm 181.7°C 94.113 g/mol Toluene -95 °C 0.87 g/mL 111 °C 92.141 g/mol Simple Filtration Column Chromatography Distilation Crystallization/Re-Crystallization Separatory funnel None www. MacBook AirBr [A] fins [B] Br [C] 17. Discuss the relationship between Compounds A, B and C. Then draw a diagram to show what you would expect to see on a TLC plate if you compared the three compounds on the same plate. 120m 9mThe protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. Standard # BSA, in mg/mL Volume of Bradford reagent, A595 in mL 1 5.0 0.000 1.00 5.0 0.134 3 2.00 5.0 0.265 4 3.00 5.0 0.388 4.00 5.0 0.497 The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413. Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.
- Consider the following data set: Sample Corrected Absorbance at 470 nm 100 ug/ml CuSO. standard 0.032 200 µg/ml Cuso. standard 0.058 300 ug/ml CusO. standard 400 µg/ml CuSO. standard 0.090 0.114 500 ug/ml CusO standard 0.140 CusO. Solution with Unknown Concentration 0.070 1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the molar absorptivity using the absorbance of the 100 ug/ml Cuso, standard. (Note: Convert ug/ml to Molarity first using the molar mass of the standard which is 249.685 g/mol). 2. Write the resulting linear regression equation and R of the plotted standard calibration curve. 3. Calculate the concentration of the "CuSO. Solution with Unknown Concentration" using the produced linear regression equation from the standard calibration curve and its absorbance at 470 nm.Is 10 ppm 400% less than 50 ppm?To run a spectrophotometry experiment, begin by warming up the spectrophotometer and preparing the samples. Be sure to select the correct absorbance (X), then run a measurement on the sample solution. Follow up by running measurements on sample solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples. Partially correct (2 of 4 correct)
- Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. XIdentify one safety violation in the picture below. Describe some safety measures to correct any violation that is presented in this photo. Answer with text and/or attachments:C8H1002 55610 170 C8H100 58466 L 160 150 140 130 120 110 100 90 80 C5H1002 59252 III 160 150 140 130 120 110 100 90 80 70 60 50 40 30 20 70 170 160 150 140 130 120 110 100 90 80 70 60 50 40 30 20 60 ppm ppm ppm