Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20. Volume of stock solution needed = mL Volume of HCl needed to adjust the imidazole group = mL Volume of HCl needed to titrate the amino group = mL Total HCl needed = mL
Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20. Volume of stock solution needed = mL Volume of HCl needed to adjust the imidazole group = mL Volume of HCl needed to titrate the amino group = mL Total HCl needed = mL
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter15: Acid–base Equilibria
Section: Chapter Questions
Problem 55P
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Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20.
Volume of stock solution needed = mL
Volume of HCl needed to adjust the imidazole group = mL
Volume of HCl needed to titrate the amino group = mL
Total HCl needed = mL
pi=8.27
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