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Q: How analytes are recovered from SFC and SFE
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A: ANSWER IS
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How are analytes usually recovered after SFC or SFE?
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- What can labs do to reduce error in GC- ms??▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAA sample of explosives residue is dissolved in an appropriate solvent and is injected into HPLC. The column contains a non- polar stationary phase. The sample revealed three chemical compounds that are commonly present in homemade explosives. ТAТP DADP HMTD 1 2 3 4 5 6 7 8 Retention time (minutes) a) determine the retention time of TATP. b) which one elute first :DADP,TATP or HMTD? c) State and explain which compound is most polar. d) If you are investigating this terrorist attacks, state one way in which the information in the chromatogram would be useful.
- How analytes are recovered from SFC and SFEuse analytical separation and analyses to outline analytical separation method protocols that can be employed to recover extremely high deposit of heavy metals and particulate matter in the river making wholesome for drinking in illegal mining communities .analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio) 1 4.47 69.58 How to get the concentration of the mixture in this case?
- ■ True or False: in a TOF analyzer, a fragment of low mass reaches the detector first ▪ In GCMS when we know what analytes we are measuring we use not know what analytes are in the sample we use than scan. ▪ A) Scan; SIM ■ B)SIM; scan • C) SIM; SIM mode. When we do mode. SIM allows greater sensitivity11. Order: Ticar (ticarcillin disodium) 1 g IVPB q6h, infuse in 50 mL D5W over 45 minutes. The instructions for the 1 g vial state to reconstitute with 2 mL of sterile water for injection yielding 1g/2.6 mL. At what rate in mL/h will you set the pump?1) Titrant 5 mg H2O/ mL Resolution 2.5 µg H2O/step Detection limit is 125 µg H2O for a 5 g sample (= 25 ppm) 2) Titrant 2 mg H2O/ mL Resolution 1 µg H2O/step Detection limit is 50 µg H2O for a 5 g sample (= 10 ppm) You have a 10 g sample which is expected to contain 200 µg H2O. True or false? 1. Titrant 5mg/mL is the preferred system over 2. Titrant 2mg/mL for this sample. True False
- Suppose that a lead determination has a standard deviation of 3 mg. If the sample has a mean value of 60 mg, the CV is 5%. For a sample containing only 15 mg, the CV iswhat is the concentration of the unknown sample using serial diluitions: 0.05M 1.7472nm, 0.025M 0.8569nm, 0.0125M 0.4167nm, 0.00625M 0.1958nm the epsilon value is 0.0101 and l is 1 cmWhy chromatography is an important tool for analysis of organic compounds?