How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why?

Q: What is the purpose of the alcohol (OH) in the gram staining process?

A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…

Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A:

Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…

A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: What’s the aim of using a microscope for examining? What can the kohler illumination technique tell…

A: A microscope is a special optical instrument that enlarges the size of the object and enables us to…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Given a log phase bacterial culture with 1 x 10^6  cells per ml and a generation time of 30 minutes…

A: Cell multiplication is due to the growth which leads to an increase in the number of bacteria over a…

Q: Would lysozyme or penicillin affect pneumoniae?

A: As per the guidelines, we are supposrd to answer only one question in case of multiple posted.…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…

A: The gram-positive and negative cells differ in their cell wall composition.

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…

A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…

Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.

A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: What technique is used to stain this bacterium? What kind of stain is used? What type of…

A: The staining is done to observe the bacteria under the microscope, there are different type of…

Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…

A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…

Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…

A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.

Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…

A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…

A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…

Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…

A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…

A: The differential stain is used to distinguish between different structures and cellular components.…

Q: The first step in identifying an unknown bacterium is to __________. culture your unknown on…

A: Bacteria are the minute microorganisms that are unicellular and have a cellular level of…

Q: In the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative…

A: Gram staining, commonly known as Gram's technique, is a staining technique that is utlised to divide…

Q: hat stain technique must this be? What is used to stain the background? What is used to stain the…

A: Capsules have a low staining ability with conventional dyes. Typically, capsule staining procedures…

Q: Which of the following microscopes produce flat images by utilizing light in the non-visual spectrum…

A: The microscope is a device used in laboratories to examine things that are hard or can not be viewed…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: to view bacterial cells and structures clearly using the light mircoscope, what object lens much be…

A: Microscopy is a technical field that concerns with the use of microscopes for viewing objects or…

Q: the chemicals used in the gram staining method?

A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…

Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…

A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…

A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…

Q: What technique is used to stain this bacterium? What kind of stain is used? What type of…

A: In this question we have to describe about one of the most important microbiology stain. Please see…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: What is acid fast staining and why do we use gram staining more?

A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…

Q: Describe the two general types of fixation. Which would you use when Gram staining a bacterium?…

A: Answer: Introduction: Fixation is a mechanism which preserves the assembly of a sample in place.

Q: What is resolution and how is it improved? With which objective is it permissible to use the course…

A: Using the fine adjustment knob which is inside the coarse adjustment knob and is used to bring the…

Q: What is the difference between gram positive and gram negative bacteria? Describe how we use this to…

A: The following are the important differences between gram-positive and gram-negative bacteria:…

Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…

A:

Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Q: After clicking the objective into position, which adjustment knob(s) can you use to safely refocus…

A: Question - (1.) - After clicking the objective into position, which adjustment knob(s) can you use…

Q: Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram…

A: The Gram staining procedure in bacteria is depends on the chemical properties of cell wall. In Gram…

Q: For each of the following stains, list each of the chemicals used (in order), briefly describe what…

A: In Microbiology staining is needed in order to properly visualize the internal membranes of the…