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- While conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.Can you show the step by step process of Methyl Red test using a schematic diagram?Why are specimens for bilirubin testing protected from light? Discuss handling procedures for bilirubin samples.
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stainFor his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar.
- Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.Does the gram staining method still have a place in today's world of advanced diagnostic methods? If yes or no, why is that so?Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.
- Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the blood agar plates?With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…